Influence of clinical factors on the protective or deleterious impact of genetic variants in orthodontically induced external root resorption: an observational study

Henriqueta Coimbra Silva; Nuno Lavado; Filomena Canova; Miguel Guevara Lopez; Fernando J Regateiro; Sónia A Pereira

doi:10.1186/s12903-022-02306-y

Influence of clinical factors on the protective or deleterious impact of genetic variants in orthodontically induced external root resorption: an observational study

BMC Oral Health. 2022 Jul 4;22(1):270. doi: 10.1186/s12903-022-02306-y.

Authors

Henriqueta Coimbra Silva^{1

2}, Nuno Lavado³, Filomena Canova³, Miguel Guevara Lopez⁴, Fernando J Regateiro^{5

6}, Sónia A Pereira⁷

Affiliations

¹ Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Coimbra, Portugal. hsilva@fmed.uc.pt.
² Institute of Medical Genetics/UCGenomics, Faculty of Medicine, University of Coimbra, Pólo 3. Subunidade 1, 3º andar, gab 77. Azinhaga de Sta Comba, Celas, 3000-548, Coimbra, Portugal. hsilva@fmed.uc.pt.
³ Polytechnic of Coimbra, Institute of Engineering of Coimbra - ISEC, Coimbra, Portugal.
⁴ Department of Computing and Systems, Setúbal School of Technology, Polytechnic Institute of Setúbal, Campus de Estefanilha, Setúbal, Portugal.
⁵ Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
⁶ Institute of Medical Genetics/UCGenomics, Faculty of Medicine, University of Coimbra, Pólo 3. Subunidade 1, 3º andar, gab 77. Azinhaga de Sta Comba, Celas, 3000-548, Coimbra, Portugal.
⁷ Institute of Orthodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.

Abstract

Background: Prediction of susceptibility to Orthodontically Induced External Apical Root Resorption (OIEARR) has been hampered by the complex architecture of this multifactorial phenotype. The aim of this study was to analyze the impact of the interaction of multiple variables in the susceptibility to OIEARR.

Methods: The study evaluated 195 patients requiring orthodontic treatment. Nine clinical and treatment variables, single nucleotide polymorphisms (SNPs) from five genes and variables interactions were analyzed as risk factors for OIEARR using a multiple linear regression model.

Results: The model explained 29% of OIEARR variability (ANOVA: p < 0.01). Duration of treatment was the most important predictor and gender was the second, closely followed by premolar extraction. For genes encoding osteoprotegerin (OPG), the receptor activator of nuclear factor κ B (RANK) and the IL1 receptor antagonist (IL1RN), the effect of analyzed variants changed from protective to deleterious depending on the duration of treatment and the age of the patient.

Conclusions: This work shows that in OIEARR the impact of genetic susceptibility factors is dynamic changing according to clinical variables.

Keywords: Complex traits; External apical root resorption; IL1RN; Orthodontics; P2RX7.

Publication types

Observational Study
Research Support, Non-U.S. Gov't

MeSH terms

Genetic Predisposition to Disease / genetics
Humans
Linear Models
Polymorphism, Single Nucleotide / genetics
Root Resorption* / genetics