Fibronectin anchoring to viscoelastic poly(dimethylsiloxane) elastomers controls fibroblast mechanosensing and directional motility

Dimitris Missirlis; Lara Heckmann; Tamás Haraszti; Joachim P Spatz

doi:10.1016/j.biomaterials.2022.121646

Fibronectin anchoring to viscoelastic poly(dimethylsiloxane) elastomers controls fibroblast mechanosensing and directional motility

Biomaterials. 2022 Aug:287:121646. doi: 10.1016/j.biomaterials.2022.121646. Epub 2022 Jun 27.

Authors

Dimitris Missirlis¹, Lara Heckmann², Tamás Haraszti³, Joachim P Spatz⁴

Affiliations

¹ Department of Cellular Biophysics, Max Planck Institute for Medical Research, Postal Address: Jahnstr. 29, D-69120, Heidelberg, Germany. Electronic address: Dimitris.Missirlis@mr.mpg.de.
² Department of Cellular Biophysics, Max Planck Institute for Medical Research, Postal Address: Jahnstr. 29, D-69120, Heidelberg, Germany.
³ DWI - Leibniz Institute for Interactive Materials, Postal Address: Forkenbeckstr. 50, D-52056, Aachen, Germany.
⁴ Department of Cellular Biophysics, Max Planck Institute for Medical Research, Postal Address: Jahnstr. 29, D-69120, Heidelberg, Germany; Department of Biophysical Chemistry, Physical Chemistry Institute, Heidelberg University, Postal Address: INF 253, D-69120, Heidelberg, Germany.

PMID: 35785752
DOI: 10.1016/j.biomaterials.2022.121646

Abstract

The established link between deregulated tissue mechanics and various pathological states calls for the elucidation of the processes through which cells interrogate and interpret the mechanical properties of their microenvironment. In this work, we demonstrate that changes in the presentation of the extracellular matrix protein fibronectin on the surface of viscoelastic silicone elastomers have an overarching effect on cell mechanosensing, that is independent of bulk mechanics. Reduction of surface hydrophilicity resulted in altered fibronectin adsorption strength as monitored using atomic force microscopy imaging and pulling experiments. Consequently, primary human fibroblasts were able to remodel the fibronectin coating, adopt a polarized phenotype and migrate directionally even on soft elastomers, that otherwise were not able to resist the applied traction forces. The findings presented here provide valuable insight on how cellular forces are regulated by ligand presentation and used by cells to probe their mechanical environment, and have implications on biomaterial design for cell guidance.