Efficacy of Diode Laser Ablation and Low Level Laser Therapy on Healing and Bacterial Load Reduction at Intraoral Biopsy Site

Aayushi Gaur; Manu Dhillon; Nidhi Puri; Upasana Sethi; Shivangi Singh; Seema Ahuja

doi:10.30476/DENTJODS.2021.88696.1354

Efficacy of Diode Laser Ablation and Low Level Laser Therapy on Healing and Bacterial Load Reduction at Intraoral Biopsy Site

J Dent (Shiraz). 2022 Jun;23(2):121-128. doi: 10.30476/DENTJODS.2021.88696.1354.

Authors

Aayushi Gaur¹, Manu Dhillon², Nidhi Puri³, Upasana Sethi³, Shivangi Singh³, Seema Ahuja³

Affiliations

¹ Postgraduate, Dept. of Oral Medicine and Radiology, I.T.S Centre for Dental Studies and Research, Muradnagar, India.
² Dept. of Oral Medicine and Radiology, Divya Jyoti Dental College, Modinagar, India.
³ Dept. of Oral Medicine and Radiology, I.T.S Centre for Dental Studies and Research, Muradnagar, India.

Abstract

Statement of the problem: Healing complications after the conventional surgical biopsy procedure along with bacterial colonization indicates scope for sophisticated techniques. Amalgamation of photo-disinfection along with healing properties of diode laser with practiced biopsy technique can help in dealing with post biopsy complications.

Purpose: The present study will analyze the possibility of conjugation of conventional surgical biopsy technique with diode laser regarding its superior properties for achieving better healing and analgesia along with sterilization of the biopsy site.

Materials and method: A randomized control trial was done where punch biopsy procedure was performed for homogenous leukoplakia. Patients were randomly divided into laser group (Test group) and control group. Test group received laser ablation and low level laser therapy (LLLT) on surgical site along with warm saline rinses whereas control group was prescribed with systemic analgesic and antibiotics. Pain on visual analogue scale (VAS), erythema along with the size of defect was evaluated on day 0, 2 and 4. Swabs were collected from the biopsy site and culture was done for evaluation of bacterial load.

Results: Highly statistical significant values indicating laser induced analgesia were obtained after analysis for 2nd and 4th day (p= 0.00). Erythema and biopsy defect size evaluation showed significant results for 2nd day (p value 0.023 and 0.004 respectively), which showed absence of erythema and enhancement of healing in test group compared to controls. Statistical significant results were obtained for estimation of bacterial colonization with p value as 0.00, 0.00 for 2nd and 4th day claiming laser supported bacterial disinfection. There was a significant percentage increase on 2nd (p= 0.013) and decrease on 4th post-operative day (p= 0.022).

Conclusion: The results encourage the conjugation of conventional incisional punch biopsy with low level lasers to avoid systemic intervention for post biopsy complications.

Keywords: Ablation; Analgesia; Bacterial load; Biopsy; Diode laser; Healing.