ST8SIA6-AS1 Promotes the Epithelial-to-Mesenchymal Transition and Angiogenesis of Pituitary Adenoma

Zuowei Li; Chengcheng Zhang; Xin Zong; Zhao Wang; Rong Ren; Lei Wang; Ping Sun; Chunmei Zhu; Mingxia Guo; Guizhen Guo; Guo Hu; Ya'nan Wu

doi:10.1155/2022/7960261

ST8SIA6-AS1 Promotes the Epithelial-to-Mesenchymal Transition and Angiogenesis of Pituitary Adenoma

J Oncol. 2022 Jun 22:2022:7960261. doi: 10.1155/2022/7960261. eCollection 2022.

Authors

Zuowei Li^{1

2}, Chengcheng Zhang¹, Xin Zong³, Zhao Wang³, Rong Ren⁴, Lei Wang⁵, Ping Sun⁴, Chunmei Zhu⁴, Mingxia Guo⁴, Guizhen Guo⁴, Guo Hu⁴, Ya'nan Wu⁴

Affiliations

¹ Shandong University of Traditional Chinese Medicine, Jinan, 250000 Shandong, China.
² Department of Encephalopathy, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250000 Shandong, China.
³ Health Management Center, People's Hospital of Chengyang, Qingdao, 266109 Shandong, China.
⁴ Department of Traditional Chinese Medicine, People' Hospital of Chengyang, Qingdao, 266109 Shandong, China.
⁵ Department of Oncology, Chengyang District Peoples Hospital, Qingdao, 266000 Shandong, China.

Abstract

To investigate the effect of long noncoding RNA ST8SIA6-AS1 on the epithelial-to-mesenchymal transition (EMT) and angiogenesis of pituitary adenoma and its possible mechanism. The expression levels of ST8SIA6-AS1 and HOXA9 in noninvasive pituitary adenoma and invasive pituitary adenoma were detected using qRT-PCR. sh-ST8SIA6-AS1 transfection silenced the expression of ST8SIA6-AS1 in GH3 and GTI-1 cells. The effects of ST8SIA6-AS1 on the proliferation, invasion, angiogenesis, and EMT of GH3 and GTI-1 pituitary adenoma cells were detected. The migration ability of cells was detected through scratch assay. Dual luciferase analysis verified the targeting relationship between ST8SIA6-AS1 and miR-5195-3p. ST8SIA6-AS1 and HOXA9 were highly expressed in invasive pituitary adenoma. In pituitary adenomas, miR-5195-3p directly targeted HOXA9. miR-5195-3p is the target gene of ST8SIA6-AS1. ST8SIA6-AS1 knockdown inhibited the proliferation, invasion, angiogenesis, and EMT of pituitary adenoma. HOXA9 expression mediates the biological effect of ST8SIA6-AS1. ST8SIA6-AS1 targets miR-5195-3p to regulate the expression of HOXA9 and promote the EMT of pituitary adenomas.