Sequencing and characterization of the chloroplast genome of Aconitum forrestii Stapf provide insights into phylogenetics in Aconitum

Mei-Hua Yang; Cong-Long Xia; Yun-Hui Guan; Hai-Zhu Zhang; Xu-Bing Chen; Ying Wang

doi:10.1080/23802359.2022.2088307

Sequencing and characterization of the chloroplast genome of Aconitum forrestii Stapf provide insights into phylogenetics in Aconitum

Mitochondrial DNA B Resour. 2022 Jun 24;7(6):1165-1167. doi: 10.1080/23802359.2022.2088307. eCollection 2022.

Authors

Mei-Hua Yang^{1

2}, Cong-Long Xia^{1

2}, Yun-Hui Guan^{1

2}, Hai-Zhu Zhang^{1

2}, Xu-Bing Chen^{1

2}, Ying Wang^{1

2}

Affiliations

¹ College of Pharmacy, Dali University, Dali, China.
² Key Laboratory of Yunnan Provincial Higher Education Institutions for Development of Yunnan Daodi Medicinal Materials Resources, Dali, China.

Abstract

Aconitum forrestii Stapf is an essential traditional Chinese medicine, and is beneficial in dispelling wind, removing dampness, warming, and relieving pain. However, its phylogenetic position of Aconitum is not accepted yet. In order to clarify the evolutionary relationship of A. forrestii, complete sequencing of chloroplast genome was carried out using Illumina sequencing technology. In total, the chloroplast genome was about 155,869 base pair (bp) in length and carried a typical tetrad structure that included a large single-copy, a small-single copy and two inverted repeat regions. A total of 132 genes were annotated, that included 85 protein -coding genes, 37 transfer RNA genes, eight ribosomal RNA genes, and two pseudogenes. The phylogenetic tree analysis indicated that Aconitum forrestii is closely related to Aconitum episcopale and Aconitum delavayi.

Keywords: Aconitum forrestii; chloroplast genome; phylogenetic analysis.

Grants and funding

This work was supported by the Major Projects of Science and Technology Plan of Dali state [D2019NA03] and Li Jian Expert Workstation of Yunnan Province [2020-1].