A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

Yiming Zhang; Brian J DeBosch

doi:10.1016/j.xpro.2022.101489

A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

STAR Protoc. 2022 Sep 16;3(3):101489. doi: 10.1016/j.xpro.2022.101489. Epub 2022 Jun 23.

Authors

Yiming Zhang¹, Brian J DeBosch²

Affiliations

¹ Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: yiming.zhang@wustl.edu.
² Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Cell Biology & Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: deboschb@wustl.edu.

Abstract

Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).

Keywords: Metabolism; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Animals
Arginine / metabolism
Autophagy*
Energy Metabolism
Humans
Hydrolases* / metabolism
Mice
Polyethylene Glycols

Substances

Polyethylene Glycols
Arginine
Hydrolases
ADI PEG20

Abstract

Publication types

MeSH terms

Substances

Grants and funding