Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease

Debbie Watson; Sam R Adhikary; Peter Cuthbertson; Nicholas J Geraghty; Katrina M Bird; Amal Elhage; Chloe Sligar; Ronald Sluyter

doi:10.1007/978-1-0716-2384-8_18

Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease

Methods Mol Biol. 2022:2510:315-340. doi: 10.1007/978-1-0716-2384-8_18.

Authors

Debbie Watson^{1

2}, Sam R Adhikary^{3

4

5}, Peter Cuthbertson^{3

4}, Nicholas J Geraghty^{3

4}, Katrina M Bird^{3

4}, Amal Elhage^{3

4}, Chloe Sligar^{3

4}, Ronald Sluyter^{3

4}

Affiliations

¹ Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia. dwatson@uow.edu.au.
² Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia. dwatson@uow.edu.au.
³ Illawarra Health and Medical Research Institute, Wollongong, NSW, Australia.
⁴ Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, Australia.
⁵ QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.

PMID: 35776334
DOI: 10.1007/978-1-0716-2384-8_18

Abstract

Humanized mouse models of graft-versus-host disease (GVHD), where human immune cells are injected into immune deficient mice, are well established and provide opportunities to investigate pathways involved in GVHD development. This chapter provides an overview of human immune cell isolation, injection of these cells into immune deficient mice, monitoring of mice for signs of GVHD, and assessment of human cell engraftment using flow cytometry. Further, this chapter focuses on the P2X7 signaling pathway involved in GVHD, and describes a strategy to block the P2X7 receptor and examine the effect of this on GVHD development.

Keywords: Flow cytometry; Hematopoietic stem cell transplantation; Human peripheral blood mononuclear cells; NSG mice; P2X7; Xenogeneic graft-versus-host disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Separation
Disease Models, Animal
Flow Cytometry
Graft vs Host Disease* / etiology
Mice
Receptors, Purinergic P2X7* / genetics

Substances

Receptors, Purinergic P2X7