The Diversity of Soil Bacteria and Fungi Under Altered Nitrogen and Rainfall Patterns in a Temperate Steppe

Yang Yu; Lu Liu; Jianing Zhao; Shuchen Wang; Yijun Zhou; Chunwang Xiao

doi:10.3389/fmicb.2022.906818

The Diversity of Soil Bacteria and Fungi Under Altered Nitrogen and Rainfall Patterns in a Temperate Steppe

Front Microbiol. 2022 Jun 14:13:906818. doi: 10.3389/fmicb.2022.906818. eCollection 2022.

Authors

Yang Yu^{1

2}, Lu Liu^{1

2}, Jianing Zhao^{1

2}, Shuchen Wang^{1

2}, Yijun Zhou^{1

2}, Chunwang Xiao^{1

2}

Affiliations

¹ Key Laboratory of Ecology and Environment in Minority Areas, Minzu University of China, National Ethnic Affairs Commission, Beijing, China.
² College of Life and Environmental Sciences, Minzu University of China, Beijing, China.

Abstract

The response of soil microorganisms to altered nitrogen (N) and rainfall patterns plays an important role in understanding ecosystem carbon and nitrogen cycling processes under global change. Previous studies have separately focused on the effects of N addition and rainfall on soil microbial diversity and community composition. However, the combined and interactive impact of N addition and rainfall on soil microbial diversity and function mediated by plant and soil processes have been poorly investigated for grassland ecosystems. Here, we conducted a field experiment with simulated N addition (N addition: 10 g N m^-2yr^-1) and altered rainfall pattern [control, rainfall reduction (compared to control -50%); rainfall addition (compared to control + 50%)] to study their interactive effects on soil microbial diversity and function in a temperate steppe of Inner Mongolia. Our results showed that N addition and rainfall addition significantly increased soil bacterial diversity, and the bacterial diversity was positively correlated with soil microbial biomass nitrogen, inorganic nitrogen, and Stipa krylovii root exudate C:N ratio, Allium polyrhizum root exudate C and N, and A. polyrhizum root exudate C:N ratio. N addition and rainfall reduction significantly reduced fungal diversity, which correlated closely with soil microbial biomass carbon and the C:N ratio of A. polyrhizum root exudates. Bacteria were mainly eutrophic r-strategists, and the responses of bacterial function guilds to the interaction between N addition and rainfall pattern were not significant. However, the arbuscular mycorrhizal fungi (AMF), in the functional classification of fungi, were significantly reduced under the condition of N addition and rainfall reduction, and the absolute abundance of the phylum Glomeromycota increased under rainfall addition, suggesting that AMFs are sensitive to altered N and rainfall patterns over short timescales (1 year). Collectively, our results have important implications for understanding the plant-soil-microbe system of grasslands under climate change.

Keywords: bacterial diversity; functional prediction; fungal diversity; nitrogen addition; rainfall addition; rainfall reduction; temperate steppe.