Performance and mechanisms exploration of nano zinc oxide (nZnO) on anaerobic decolorization by Shewanella oneidensis MR-1

Feng-Ting Cao; Xiao-Lin Ma; Xiang-Tong Zhou; Jun-Cheng Han; Xiang Xiao

doi:10.1016/j.chemosphere.2022.135510

Performance and mechanisms exploration of nano zinc oxide (nZnO) on anaerobic decolorization by Shewanella oneidensis MR-1

Chemosphere. 2022 Oct:305:135510. doi: 10.1016/j.chemosphere.2022.135510. Epub 2022 Jun 27.

Authors

Feng-Ting Cao¹, Xiao-Lin Ma², Xiang-Tong Zhou³, Jun-Cheng Han⁴, Xiang Xiao⁵

Affiliations

¹ Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, China.
² Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, China; School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
³ School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
⁴ Department of Civil and Environmental Engineering, School of Civil and Environmental Engineering, Ningbo University, Ningbo, 315211, China.
⁵ Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institutes of Physical Science and Information Technology, Anhui University, Hefei, 230601, China; School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China. Electronic address: xiaox@ustc.edu.cn.

PMID: 35772516
DOI: 10.1016/j.chemosphere.2022.135510

Abstract

Although the ecological safety of nanomaterials is of widespread concern, their current ambient concentrations are not yet sufficient to cause serious toxic effects. Thus, the nontoxic bioimpact of nanomaterials in wastewater treatment has attracted increasing attention. In this study, the effect of nano zinc oxide (nZnO), one of the most widely used nanomaterials, on the anaerobic biodegradation of methyl orange (MO) by Shewanella oneidensis MR-1 was comprehensively investigated. High-dosage nZnO (>0.5 mg/L) caused severe toxic stress on S. oneidensis MR-1, resulting in the decrease in decolorization efficiency. However, nZnO at ambient concentrations could act as nanostimulants and promote the anaerobic removal of MO by S. oneidensis MR-1, which should be attributed to the improvement of decolorization efficiency rather than cell proliferation. The dissolved Zn²⁺ was found to contribute to the bioeffect of nZnO on MO decolorization. Further investigation revealed that low-dosage nZnO could promote the cell viability, membrane permeability, anaerobic metabolism, as well as related gene expression, indicating that nZnO facilitated rather than inhibited the anaerobic wastewater treatment under ambient conditions. Thus, this work provides a new insight into the bioeffect of nZnO in actual environment and facilitates the practical application of nanomaterials as nanostimulants in biological process.

Keywords: Anaerobic decolorization; Biotreatment; S. oneidensis MR-1; Stimulatory effect; nZnO.

MeSH terms

Anaerobiosis
Biodegradation, Environmental
Shewanella* / metabolism
Zinc Oxide* / metabolism
Zinc Oxide* / toxicity

Substances

Zinc Oxide

Supplementary concepts

Shewanella oneidensis