Highly sensitive and convenient aptasensor based on Au NPs@Ce-TpBpy COF for quantitative determination of zearalenone

Zhixiong Chen; Meng Yang; Ziying Li; Wenchun Liao; Bengqi Chen; Tong Yang; Rong Hu; Yunhui Yang; Shuang Meng

doi:10.1039/d2ra02093a

Highly sensitive and convenient aptasensor based on Au NPs@Ce-TpBpy COF for quantitative determination of zearalenone

RSC Adv. 2022 Jun 13;12(27):17312-17320. doi: 10.1039/d2ra02093a. eCollection 2022 Jun 7.

Authors

Zhixiong Chen¹, Meng Yang¹, Ziying Li¹, Wenchun Liao¹, Bengqi Chen¹, Tong Yang¹, Rong Hu¹, Yunhui Yang¹, Shuang Meng¹

Affiliation

¹ College of Chemistry and Chemical Engineering, Yunnan Normal University Kunming 650500 China yyhui2002@aliyun.com ms19880719@126.com +86 871 65941086.

Abstract

In this work, an aptasensor based on a portable U-disk electrochemical workstation in combination with a screen-printed electrode (SPE) is demonstrated for the quantitative determination of zearalenone (ZEN). The aptamer is immobilized on Au NPs@Ce-TpBpy COF (Covalent organic frameworks), which is modified on the surface of glassy carbon electrode. ZEN specifically binds to ZEN aptamer, which hinders the electron transfer and decreases the catalytic current of Au NPs@Ce-TpBpy COF for the reduction of hydrogen peroxide, measured by chronoamperometry (i-t). The quantitative detection of ZEN toxin is realized by a decrease of the catalytic current (ΔI). Under the optimal experimental conditions, the aptamer sensor exhibited excellent sensitivity, selectivity, reproducibility. A wide linear range of 1 pg mL^-1-10.0 ng mL^-1 with a detection limit of 0.389 pg mL^-1 (at 3σ) was obtained. The linear equation is ΔI = 0.401 lg c + 1.948 with a correlation coefficient of 0.9906. The recovery is in the range of 93.0-104.7% for the cornflour samples. The proposed method offers a new strategy for the rapid, inexpensive, and real-time detection of ZEN.