The protective mechanism of extracellular polymeric substance (EPS) secreted by a harmful cyanobacteria against tannins allelochemicals was explored in this study. The binding properties of soluble EPS (SEPS) and bound EPS (BEPS) of Microcystis aeruginosa to tannic acid (TA) were investigated via fluorescence spectroscopy. The results suggested that TA interacted with the proteins in SEPS and BEPS mainly with binding constants of 5.26 and 7.93 L/mol, respectively; TA interacted with the humic acids in SEPS and BEPS mainly with binding constants of 5.12 and 5.24 L/mol, respectively. Thermodynamic experiments confirmed that the binding was mainly controlled by the hydrophobic force. Combined with Fourier transform infrared spectroscopy, it was found that the amine, carbonyl, carboxyl, and hydroxyl groups in EPS were the main functional groups contributing to the interaction of TA with EPS. The existence of EPS reduced the toxicity of TA to algal cells, with the 96 h inhibition rate of 40 mg L-1 TA on algal cells decreasing by 48.95%. The results of this study may improve our understanding of the protective mechanism of cyanobacteria against tannins allelochemicals.
Keywords: Cyanobacteria; Extracellular polymeric substance; Interactions; Microcystis aeruginosa; Tannic acid; Tannins.
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.