SCaBP3/CBL7 negatively regulates the plasma membrane H+-ATPase and modulates hypocotyl elongation in Arabidopsis

Plant Signal Behav. 2022 Dec 31;17(1):2092699. doi: 10.1080/15592324.2022.2092699.

Abstract

The regulation of hypocotyl elongation is an important process in plant growth and development and depends on the activity of the plasma membrane (PM) H+-ATPase. In this study, we found that the Arabidopsis protein SOS3-LIKE CALCIUM BINDING PROTEIN3 (SCaBP3) negatively regulates PM H+-ATPase activity in yeast and hypocotyl elongation in dark-grown seedlings. Yeast two-hybrid assays showed that SCaBP3 interacts with representative members of the Arabidopsis PM H+-ATPase family. Experiments in RS-72 yeast showed that SCaBP3 negatively regulates PM H+-ATPase activity-dependent yeast cell growth. Hypocotyl elongation was promoted in the loss-of-function mutant scabp3 and inhibited in SCaBP3 overexpression lines of Arabidopsis. We propose that SCaBP3 modulates hypocotyl elongation by negatively regulating PM H+-ATPase activity.

Keywords: Arabidopsis thaliana; PM H+-ATPase; calcium binding protein SCaBP3; hypocotyl elongation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / genetics
  • Arabidopsis* / metabolism
  • Calcium-Binding Proteins* / genetics
  • Calcium-Binding Proteins* / metabolism
  • Cell Membrane / metabolism
  • Gene Expression Regulation, Plant
  • Hypocotyl / growth & development
  • Proton-Translocating ATPases* / genetics
  • Proton-Translocating ATPases* / metabolism

Substances

  • Arabidopsis Proteins
  • CBL7 protein, Arabidopsis
  • Calcium-Binding Proteins
  • Proton-Translocating ATPases

Grants and funding

This work was supported by the National Natural Science Foundation of China (31670260, 31872659) and Exploration project of Center for Practical Courses in the Life Sciences of China Agricultural University (20210102, 20210103).