Mycorrhiza-mediated potassium transport in Medicago truncatula can be evaluated by using rubidium as a proxy

Abstract

Arbuscular mycorrhizal (AM) fungi considerably improve plant nutrient acquisition, particularly phosphorus and nitrogen. Despite the physiological importance of potassium (K⁺) in plants, there is increasing interest in the mycorrhizal contribution to plant K⁺ nutrition. Yet, methods to track K⁺ transport are often costly and limiting evaluation opportunities. Rubidium (Rb⁺) is known to be transported through same pathways as K⁺. As such our research efforts attempt to evaluate if Rb⁺ could serve as a viable proxy for evaluating K⁺ transport in AM symbiosis. Therefore, we examined the transport of K⁺ in Medicago truncatula colonized by the AM fungus Rhizophagus irregularis isolate 09 having access to various concentrations of Rb⁺ in custom-made two-compartment systems. Plant biomass, fungal root colonization, and shoot nutrient concentrations were recorded under sufficient and limited K⁺ regimes. We report that AM plants displayed higher shoot Rb⁺ and K⁺ concentrations and a greater K⁺:Na⁺ ratio relative to non-colonized plants in both sufficient and limited K⁺ conditions. Consequently, our results indicate that Rb⁺ can be used as a proxy to assess the movement of K⁺ in AM symbiosis, and suggest the existence of a mycorrhizal uptake pathway for K⁺ nutrition in M. truncatula.

Keywords: Arbuscular mycorrhizal symbiosis; Medicago truncatula; Plant nutrition; Potassium; Rhizophagus irregularis; Rubidium.