SARS-CoV-2 RNA in Wastewater Was Highly Correlated With the Number of COVID-19 Cases During the Fourth and Fifth Pandemic Wave in Kobe City, Japan

Yoshihiko Tanimoto; Erika Ito; Sonoko Miyamoto; Ai Mori; Ryohei Nomoto; Noriko Nakanishi; Naohiro Oka; Takao Morimoto; Tomotada Iwamoto

doi:10.3389/fmicb.2022.892447

SARS-CoV-2 RNA in Wastewater Was Highly Correlated With the Number of COVID-19 Cases During the Fourth and Fifth Pandemic Wave in Kobe City, Japan

Front Microbiol. 2022 Jun 9:13:892447. doi: 10.3389/fmicb.2022.892447. eCollection 2022.

Authors

Yoshihiko Tanimoto¹, Erika Ito¹, Sonoko Miyamoto¹, Ai Mori¹, Ryohei Nomoto¹, Noriko Nakanishi¹, Naohiro Oka², Takao Morimoto², Tomotada Iwamoto¹

Affiliations

¹ Department of Infectious Diseases, Kobe Institute of Health, Kobe City, Japan.
² Planning Division, Sewage Works Department, Public Construction Projects Bureau, Kobe City, Japan.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the current coronavirus disease 2019 (COVID-19) pandemic and associated respiratory infections, has been detected in the feces of patients. Therefore, determining SARS-CoV-2 RNA levels in sewage may help to predict the number of infected people within the area. In this study, we quantified SARS-CoV-2 RNA copy number using reverse transcription quantitative real-time PCR with primers and probes targeting the N gene, which allows the detection of both wild-type and variant strain of SARS-CoV-2 in sewage samples from two wastewater treatment plants (WWTPs) in Kobe City, Japan, during the fourth and fifth pandemic waves of COVID-19 between February 2021 and October 2021. The wastewater samples were concentrated via centrifugation, yielding a pelleted solid fraction and a supernatant, which was subjected to polyethylene glycol (PEG) precipitation. The SARS-CoV-2 RNA was significantly and frequently detected in the solid fraction than in the PEG-precipitated fraction. In addition, the copy number in the solid fraction was highly correlated with the number of COVID-19 cases in the WWTP basin (WWTP-A: r = 0.8205, p < 0.001; WWTP-B: r = 0.8482, p < 0.001). The limit of capturing COVID-19 cases per 100,000 people was 0.75 cases in WWTP-A and 1.20 cases in WWTP-B, respectively. Quantitative studies of RNA in sewage can be useful for administrative purposes related to public health, including issuing warnings and implementing preventive measures within sewage basins.

Keywords: COVID-19; SARS-CoV-2; environmental surveillance; sewage; wastewater.