In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism

Jingfei Yao; Dongmei Wu; Yifu Qiu

doi:10.1016/j.xpro.2022.101480

In vitro analyses of paracrine effects of murine classically activated macrophage on beige adipocyte metabolism

STAR Protoc. 2022 Jun 17;3(3):101480. doi: 10.1016/j.xpro.2022.101480. eCollection 2022 Sep 16.

Authors

Jingfei Yao¹, Dongmei Wu², Yifu Qiu³

Affiliations

¹ Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, College of Future Technology, Peking University, Beijing, China. Electronic address: yaojf16@pku.edu.cn.
² Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, College of Future Technology, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
³ Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, College of Future Technology, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China. Electronic address: yifu.qiu@pku.edu.cn.

Abstract

The communication between macrophage and adipocyte plays a critical role in the initiation and development of metabolic inflammation, which is difficult to study in vivo. Here, we provide a step-by-step protocol using differentiated cells to investigate the paracrine effects of classically activated macrophage on beige adipocyte metabolism in vitro. This protocol uses bone-marrow-derived macrophage and SVF-derived UCP1⁺ beige adipocyte in a culture model to study immune regulation of adipocyte metabolism by western blot analyses. For complete details on the use and execution of this protocol, please refer to Yao et al. (2021).

Keywords: Immunology; Metabolism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / metabolism
Adipocytes, Beige*
Animals
Cell Differentiation
Macrophages
Mice
Thermogenesis