Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction

Abstract

Due to the expansive application of TiO₂ and its variance in physico-chemical characteristics, the toxicological profile of TiO₂, in all its various forms, requires evaluation. This study aimed to assess the hazard of five TiO₂ particle-types in relation to their cytotoxic profile correlated to their cellular interaction, specifically in human lymphoblast (TK6) and type-II alveolar epithelial (A549) cells. Treatment with the test materials was undertaken at a concentration range of 1-100 μg/cm² over 24 and 72 h exposure. TiO₂ interaction with both cell types was visualised by transmission electron microscopy, supported by energy-dispersive X-ray. None of the TiO₂ materials tested promoted cytotoxicity in either cell type over the concentration and time range studied. All materials were observed to interact with the A549 cells and were further noted to be internalised following 24 h exposure. In contrast, only the pigmentary rutile was internalised by TK6 lymphoblasts after 24 h exposure. Where uptake was observed there was no evidence, as determined by 2D microscopy techniques, of particle localisation within the nucleus of either cell type. This study indicates that industrially relevant TiO₂ particles demonstrate cell interactions that are cell-type dependent and do not induce cytotoxicity at the applied dose range.

Keywords: Cellular association; Cellular uptake; Cytotoxicity; Industrially relevant; Titanium dioxide.