Synthesis of blue fluorescent molybdenum nanoclusters with novel terephthaldehyde-cysteine Schiff base for detection of pyrophosphate

Mehul R Kateshiya; Naved I Malek; Suresh Kumar Kailasa

doi:10.1016/j.saa.2022.121536

Synthesis of blue fluorescent molybdenum nanoclusters with novel terephthaldehyde-cysteine Schiff base for detection of pyrophosphate

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 5:280:121536. doi: 10.1016/j.saa.2022.121536. Epub 2022 Jun 20.

Authors

Mehul R Kateshiya¹, Naved I Malek¹, Suresh Kumar Kailasa²

Affiliations

¹ Department of Chemistry, Sardar Vallbhbhai National Institute of Technology, Surat 395 007, Gujarat, India.
² Department of Chemistry, Sardar Vallbhbhai National Institute of Technology, Surat 395 007, Gujarat, India. Electronic address: sureshkumarchem@gmail.com.

PMID: 35752042
DOI: 10.1016/j.saa.2022.121536

Abstract

In this work, terephthaldehyde-cysteine-molybdenum nanoclusters (TPA-Cys-MoNCs) were synthesized by using terephthaldehyde-cysteine (TPA-Cys) Schiff base as a novel ligand. The as-synthesized TPA-Cys-MoNCs showed blue fluorescence under UV lamp at 365 nm, displaying emission peak at 455 nm when excited at 340 nm. The fluorescent TPA-Cys-MoNCs are used as a probe for sensitive assay of pyrophosphate (PPi) via fluorescence quenching mechanism. The emission peak intensity of TPA-Cys-MoNCs at 455 nm exhibited a linear quenching with increasing amount of PPi. As a result, quantitative assay was developed for the detection of PPi (0.01-200 µM) with the detection limit of 0.9 nM. The developed probe was successfully demonstrated for the detection of PPi in biofluids (urine and plasma).

Keywords: Fluorescence assay; HR-TEM and biofluids; Pyrophosphate; TPA-Cys-MoNCs.

MeSH terms

Cysteine*
Diphosphates
Fluorescent Dyes
Limit of Detection
Molybdenum*
Schiff Bases
Spectrometry, Fluorescence

Substances

Diphosphates
Fluorescent Dyes
Schiff Bases
Molybdenum
Cysteine