Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis

Li-Jun Long; Min Lin; Yu-Ran Chen; Xin Meng; Ting-Ting Cui; Ya-Ping Li; Xu-Guang Guo

doi:10.1186/s12941-022-00522-6

Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis

Ann Clin Microbiol Antimicrob. 2022 Jun 24;21(1):27. doi: 10.1186/s12941-022-00522-6.

Authors

Li-Jun Long^#^{1

2}, Min Lin^#^{1

3}, Yu-Ran Chen^{1

2}, Xin Meng^{1

4}, Ting-Ting Cui⁵, Ya-Ping Li⁶, Xu-Guang Guo^{7

8

9

10}

Affiliations

¹ Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
² Department of Medical Laboratory Technology, The King Med School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, 511436, China.
³ Department of Traditional Chinese and Western Medicine in Clinical Medicine, The Clinical School of Traditional Chinese and Western Medicine of Guangzhou Medical University, Guangzhou, 511436, China.
⁴ Department of Medical Imageology, The Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
⁵ Department of Pediatrics, Pediatrics School, Guangzhou Medical University, Guangzhou, 511436, China.
⁶ Department of Clinical Medicine, The Second Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.
⁷ Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China. gysygxg@gmail.com.
⁸ Department of Medical Imageology, The Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China. gysygxg@gmail.com.
⁹ Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China. gysygxg@gmail.com.
¹⁰ Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China. gysygxg@gmail.com.

^# Contributed equally.

Abstract

Background: Staphylococcus aureus can cause many diseases and even death. It's important to detect Staphylococcus aureus rapidly and reliably. The accuracy of a novel test named LAMP in detecting Staphylococcus aureus is unclear. Therefore, a systematic review and meta-analysis were conducted to evaluate the accuracy of the LAMP assay for Staphylococcus aureus detection.

Methods: Four databases were searched for relevant studies. Meta-DiSc 1.4.0 and Stata 12.0 were used for statistical analysis. At the same time, we used QUADAS-2 to assess the studies we included. Two groups of subgroup analysis were done to differentiate the diagnostic effects of various LAMP tests and in cases of different gold standards.

Results: 11 studies were identified and 19 2 × 2 contingency tables were extracted in our study. The results showed that both pooled sensitivity and specificity of the LAMP assay were 99% (95% CI 99-100).

Conclusion: The LAMP assay demonstrated high sensitivity and specificity in diagnosing Staphylococcus aureus.

Keywords: Detection; Loop-mediated isothermal amplification; Methicillin-resistant Staphylococcus aureus; Staphylococcus aureus.

Publication types

Meta-Analysis
Systematic Review

MeSH terms

Humans
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques / methods
Sensitivity and Specificity
Staphylococcal Infections* / diagnosis
Staphylococcus aureus* / genetics

Supplementary concepts

LAMP assay