A standard method to assess changes in craniofacial morphology over time is through the superimposition of serial patient images. This study evaluated the reliability of a novel anterior cranial base reference area, principally including stable midline structures (EMACB) after an early age, and compared it to the total anterior cranial base (TACB) and an area including only midline structures (MACB). Fifteen pairs of pre-existing serial CBCT images acquired from growing patients were superimposed with all techniques by applying a best-fit registration algorithm of corresponding voxel intensities (Dolphin 3D software). The research outcomes were the reproducibility of each technique and the agreement between them in skeletal change detection, as well as their validity. The TACB and EMACB methods were valid, since the superimposed midline ACB structures consistently showed adequate overlap. They also presented perfect overall reproducibility (median error < 0.01 mm) and agreement (median difference < 0.01 mm). MACB showed reduced validity, higher errors, and a moderate agreement to the TACB. Thus, the EMACB method performed efficiently and mainly included the stable midline ACB structures during growth. Based on the technical, anatomical, and biological principles applied when superimposing serial 3D data to assess craniofacial changes, we recommend the EMACB method as the method of choice to fulfil this purpose.
Keywords: anterior cranial base; cone-beam computed tomography; imaging; three-dimensional; voxel-based superimposition.