Impacts of nano-titanium dioxide toward Vallisneria natans and epiphytic microbes

Abstract

In this study, Vallisneria natans plants were exposed to 5 and 20 nm of titanium dioxide nanoparticles (TiO₂ NPs) anatase and 600-1000 nm of bulk at 5 and 20 mg/L for 30 days. SEM images and EDX spectra revealed that epiphytic biofilms were more prone to TiO₂ NPs adhesion than bare plant leaves. TiO₂ NPs injured plant leaf cells, ruptured epiphytic diatoms membranes and increased the ratio of free-living microbes. The TN, NH₄⁺-N and NO₃--N concentrations significantly decreased, respectively, by 44.9%, 33.6%, and 23.6% compared to bulk treatments after 30 days due to macrophyte damage and a decline in diversity of epiphytic bacterial community and abundance of nitrogen cycle bacteria. TiO₂ NPs size-dependent decrease in bacterial relative abundance was detected, including phylum Cyanobacteria, Planctomycetes, and Verrucomicrobia. Although TiO₂ NPs increased eukaryotic diversity and abundance, abundances of Bacillariophyceae and Vampyrellidae classes and Gastrotricha and Phragmoplastophyta phylum decreased significantly under TiO₂ NPs exposure compared to bulk and control. TiO₂ NPs reduced intensities of interaction relationships among epiphytic microbial genera. This study shed new light on the potential effects of TiO₂ NPs toxicity toward aquatic plants and epiphytic microbial communities and its impacts on nitrogen species removal in wetlands.

Keywords: Epiphytic biofilm; Particle size; Titanium dioxide nanoparticles; V. natans; Wetlands.