PeTGA1 enhances disease resistance against Colletotrichum gloeosporioides through directly regulating PeSARD1 in poplar

Yanli Yang; Hui-Guang Li; Meiying Liu; Hou-Ling Wang; Qi Yang; Dong-Hui Yan; Ying Zhang; Zhonghai Li; Cong-Hua Feng; Mengxue Niu; Chao Liu; Weilun Yin; Xinli Xia

doi:10.1016/j.ijbiomac.2022.06.099

PeTGA1 enhances disease resistance against Colletotrichum gloeosporioides through directly regulating PeSARD1 in poplar

Int J Biol Macromol. 2022 Aug 1:214:672-684. doi: 10.1016/j.ijbiomac.2022.06.099. Epub 2022 Jun 20.

Authors

Yanli Yang¹, Hui-Guang Li², Meiying Liu³, Hou-Ling Wang⁴, Qi Yang⁵, Dong-Hui Yan⁶, Ying Zhang⁷, Zhonghai Li⁸, Cong-Hua Feng⁹, Mengxue Niu¹⁰, Chao Liu⁷, Weilun Yin¹¹, Xinli Xia¹²

Affiliations

¹ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: yangyl@bjfu.edu.cn.
² National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: hg_li@bjfu.edu.cn.
³ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: lmying@bjfu.edu.cn.
⁴ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: whling@bjfu.edu.cn.
⁵ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: yangqi0808@bjfu.edu.cn.
⁶ Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, The Key Laboratory of Forest Protection Affiliated to State Forestry and Grassland Administration of China, Beijing 100091, China. Electronic address: yandh@caf.ac.cn.
⁷ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
⁸ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: lizhonghai@bjfu.edu.cn.
⁹ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: fengconghua@bjfu.edu.en.
¹⁰ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: niumengxue@bjfu.edu.cn.
¹¹ National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: yinwl@bjfu.edu.cn.
¹² National Engineering Research Center of Tree breeding and Ecological remediation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: xiaxl@bjfu.edu.cn.

PMID: 35738343
DOI: 10.1016/j.ijbiomac.2022.06.099

Abstract

Basic leucine zipper (bZIP) proteins play important roles in responding to biotic and abiotic stresses in plants. However, the molecular mechanisms of plant resistance to pathogens remain largely unclear in poplar. The present study isolated a TGACG-binding (TGA) transcription factor, PeTGA1, from Populus euphratica. PeTGA1 belongs to subgroup D of the bZIP family and was localized to the nucleus. To study the role PeTGA1 plays in response to Colletotrichum gloeosporioides, transgenic triploid white poplars overexpressing PeTGA1 were generated. Results showed that poplars with overexpressed PeTGA1 showed a higher effective defense response to C. gloeosporioides than the wild-type plants. A yeast one-hybrid assay and an electrophoretic mobility shift assay revealed that PeTGA1 could directly bind to the PeSARD1 (P. euphratica SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1) promoter, an important regulator for salicylic acid biosynthesis. The transactivation assays indicated that PeTGA1 activated the expression of PeSARD1, and PR1 (PATHOGENESIS-RELATED 1), a SA marker gene involved in SA signaling. Subsequently, we observed that the PeTGA1 overexpression lines showed elevated SA levels, thereby resulting in the increased resistance to C. gloeosporioides. Taken together, our results indicated that PeTGA1 may exert a key role in plant immunity not only by targeting PeSARD1 thus participating in the SA biosynthesis pathway but also by involving in SA signaling via activating the expression of PR1.

Keywords: Colletotrichum gloeosporioides; PeSARD1; PeTGA1; Populus; Salicylic acid.

MeSH terms

Basic-Leucine Zipper Transcription Factors / genetics
Colletotrichum* / metabolism
Disease Resistance / genetics
Gene Expression Regulation, Plant
Plant Diseases / genetics
Plant Proteins / chemistry
Plants, Genetically Modified / genetics
Populus* / genetics
Populus* / metabolism
Salicylic Acid / metabolism

Substances

Basic-Leucine Zipper Transcription Factors
Plant Proteins
Salicylic Acid

Supplementary concepts

Colletotrichum gloeosporioides