The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer

Bull Exp Biol Med. 2022 Jun;173(2):261-264. doi: 10.1007/s10517-022-05530-2. Epub 2022 Jun 23.

Abstract

We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer.

Keywords: LINE-1; colorectal cancer; freely circulating RNA; long dispersed nucleotide repeats (LINE); transcription.

MeSH terms

  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics
  • Cell-Free Nucleic Acids* / blood
  • Cell-Free Nucleic Acids* / genetics
  • Colonic Neoplasms* / blood
  • Colonic Neoplasms* / genetics
  • Colorectal Neoplasms* / blood
  • Colorectal Neoplasms* / genetics
  • Colorectal Neoplasms* / pathology
  • Humans
  • RNA, Messenger / blood
  • RNA, Messenger / genetics
  • RNA-Binding Proteins* / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Biomarkers, Tumor
  • Cell-Free Nucleic Acids
  • RNA, Messenger
  • RNA-Binding Proteins