Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1

Huhe Chao; Haohai Ma; Jiadong Sun; Shuai Yuan; Peiyu Dong; Aihong Zhao; Lan Li; Wei Shen; Xifeng Zhang

doi:10.3390/toxins14060373

Whole-Transcriptome Analysis of Non-Coding RNA Alteration in Porcine Alveolar Macrophage Exposed to Aflatoxin B1

Toxins (Basel). 2022 May 27;14(6):373. doi: 10.3390/toxins14060373.

Authors

Huhe Chao^{1

2}, Haohai Ma¹, Jiadong Sun³, Shuai Yuan⁴, Peiyu Dong¹, Aihong Zhao⁵, Lan Li³, Wei Shen³, Xifeng Zhang¹

Affiliations

¹ College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
² Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100023, China.
³ Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
⁴ School of Medicine, Henan Polytechnic University, Jiaozuo 454000, China.
⁵ Qingdao Academy of Agricultural Science, Qingdao 266100, China.

Abstract

Aflatoxin B1 (AFB1) is a type of mycotoxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus and is commonly found in cereals, oils and foodstuffs. In order to understand the toxic effects of AFB1 exposure on Porcine alveolar macrophages (3D4/2 cell), the 3D4/2 cells were exposed to 40 μg/mL AFB1 for 24 h in vitro, and several methods were used for analysis. Edu and TUNEL analysis showed that the proliferation of 3D4/2 cells was significantly inhibited and the apoptosis of 3D4/2 cells was significantly induced after AFB1 exposure compared with that of the control group. Whole-transcriptome analysis was performed to reveal the non-coding RNA alteration in 3D4/2 cells after AFB1 exposure. It was found that the expression of cell-cycle-related and apoptosis-related genes was altered after AFB1 exposure, and lncRNAs and miRNAs were also significantly different among the experimental groups. In particular, AFB1 exposure affected the expression of lncRNAs associated with cellular senescence signaling pathways, such as MSTRG.24315 and MSTRG.80767, as well as related genes, Cxcl8 and Gadd45g. In addition, AFB1 exposure affected the expression of miRNAs associated with immune-related genes, such as miR-181a, miR-331-3p and miR-342, as well as immune-related genes Nfkb1 and Rras2. Moreover, the regulation networks between mRNA-miRNAs and mRNA-lncRNAs were confirmed by the results of RT-qPCR and immunofluorescence. In conclusion, our results here demonstrate that AFB1 exposure impaired proliferation of 3D4/2 cells via the non-coding RNA-mediated pathway.

Keywords: aflatoxin B1; apoptosis; cell cycle; porcine; porcine alveolar macrophages.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aflatoxin B1 / toxicity
Animals
Gene Expression Profiling
Macrophages, Alveolar
MicroRNAs* / genetics
RNA, Long Noncoding* / genetics
RNA, Messenger
Swine

Substances

MicroRNAs
RNA, Long Noncoding
RNA, Messenger
Aflatoxin B1

Grants and funding

This work was supported by High level talents research fund project of Qingdao Agricultural University in China (1120043) to Zhang XF, Science & Technology Fund Planning Projects of Qingdao City (21-1-4-ny-7-nsh), the Natural Science Foundation of Shandong Province of China (ZR2021MC191), the Taishan Scholar Construction Foundation of Shandong province of China (ts20190946), Gene expression analysis of hormone receptor-negative breast cancer with low HER-2 expression and its potential influence on neoadjuvant chemotherapy (212102310658), the Cultivating Fund of Capital Medical University (Grant No. PYZ2017002).