Target ischemic stroke model creation method using photoacoustic microscopy with simultaneous vessel monitoring and dynamic photothrombosis induction

Daewoon Seong; Soojin Yi; Sangyeob Han; Jaeyul Lee; Sungjo Park; Yang-Ha Hwang; Jeehyun Kim; Hong Kyun Kim; Mansik Jeon

doi:10.1016/j.pacs.2022.100376

Target ischemic stroke model creation method using photoacoustic microscopy with simultaneous vessel monitoring and dynamic photothrombosis induction

Photoacoustics. 2022 Jun 6:27:100376. doi: 10.1016/j.pacs.2022.100376. eCollection 2022 Sep.

Authors

Daewoon Seong¹, Soojin Yi^{2

3

4}, Sangyeob Han^{1

5}, Jaeyul Lee^{1

6}, Sungjo Park⁷, Yang-Ha Hwang⁸, Jeehyun Kim¹, Hong Kyun Kim^{2

3

4}, Mansik Jeon¹

Affiliations

¹ School of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, the Republic of Korea.
² Bio-Medical Institute, Kyungpook National University Hospital, Daegu 41404, the Republic of Korea.
³ Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu 41944, the Republic of Korea.
⁴ Department of Biomedical Science, The Graduate School, Kyungpook National University, Daegu 41944, the Republic of Korea.
⁵ Institute of Biomedical Engineering, School of Medicine, Kyungpook National University, Daegu 41566, the Republic of Korea.
⁶ Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
⁷ Pohang Innotown Center, Pohang University of Science and Technology, Pohang 37673, the Republic of Korea.
⁸ Department of Neurology, School of Medicine, Kyungpook National University, Daegu 41944, the Republic of Korea.

Abstract

The ischemic stroke animal model evaluates the efficacy of reperfusion and neuroprotective strategies for ischemic injuries. Various conventional methods have been reported to induce the ischemic models; however, controlling specific neurological deficits, mortality rates, and the extent of the infarction is difficult as the size of the affected region is not precisely controlled. In this paper, we report a single laser-based localized target ischemic stroke model development method by simultaneous vessel monitoring and photothrombosis induction using photoacoustic microscopy (PAM), which has minimized the infarct size at precise location with high reproducibility. The proposed method has significantly reduced the infarcted region by illuminating the precise localization. The reproducibility and validity of suggested method have been demonstrated through repeated experiments and histological analyses. These results demonstrate that our method can provide the ischemic stroke model closest to the clinical pathology for brain ischemia research from inducement, occurrence mechanisms to the recovery process.

Keywords: Dynamic photothrombosis induction; Localized target ischemic stroke model; Minimal invasive model; Photoacoustic microscopy; Target blood vessel monitoring.