Bioinformatics Methods for ChIP-seq Histone Analysis

Abstract

The field of genomics and genome-wide analysis has exploded since around 2008 with the development of high-throughput omics approaches, largely driven by the emergence of the next-generation sequencing technologies. Among the different biological applications supported by recent sequencing technologies, ChIP-seq (Chromatin ImmunoPrecipitation followed by Sequencing) is one of the most powerful techniques which has dramatically changed our view of the epigenetics landscape of cells.In this chapter, I will present and discuss the main steps of bioinformatic and biostatistical analysis of ChIP-seq data (Fig. 1). While this technique has been widely used to study transcription factor binding sites, I will focus here on the analysis of histone modifications.

Keywords: Bioinformatics; ChIP-seq; Differential binding; Peak calling; Quality controls.