Agrochemical control of gene expression using evolved split RNA polymerase

Yuan Yuan; Jin Miao

doi:10.7717/peerj.13619

Agrochemical control of gene expression using evolved split RNA polymerase

PeerJ. 2022 Jun 16:10:e13619. doi: 10.7717/peerj.13619. eCollection 2022.

Authors

Yuan Yuan¹, Jin Miao²

Affiliations

¹ Department of Neurophysiology and Neuropharmacology, Institute of Special Environmental Medicine and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
² Duke Kunshan University, Kunshan, China.

Abstract

Chemically-inducible gene expression systems are valuable tools for rational control of gene expression both for basic research and biotechnology. However, most chemical inducers are confined to certain groups of organisms. Therefore, dissecting interactions between different organisms could be challenging using existing chemically-inducible systems. We engineered a mandipropamid-induced gene expression system (Mandi-T7) based on evolved split T7 RNAP system. As a proof-of-principle, we induced GFP expression in E. coli cells grown inside plant tissue.

Keywords: Inducible gene expression; Mandipropamid; T7 RNAP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotechnology
DNA-Directed RNA Polymerases / genetics
Escherichia coli* / genetics
Gene Expression Regulation, Bacterial* / drug effects
Gene Expression Regulation, Bacterial* / genetics
Gene Expression* / drug effects
Gene Expression* / genetics
Plants* / microbiology
Viral Proteins / chemistry

Substances

DNA-Directed RNA Polymerases
Viral Proteins
mandipropamid

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant 31500690 to Jin Miao). There was no additional external funding received for this study.The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.