Reporter Systems for Assessments of Extracellular Vesicle Transfer

Chaoshan Han; Gangjian Qin

doi:10.3389/fcvm.2022.922420

Reporter Systems for Assessments of Extracellular Vesicle Transfer

Front Cardiovasc Med. 2022 Jun 1:9:922420. doi: 10.3389/fcvm.2022.922420. eCollection 2022.

Authors

Chaoshan Han^{1

2}, Gangjian Qin^{1

2}

Affiliations

¹ Department of Biomedical Engineering, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.
² Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, Birmingham, AL, United States.

Abstract

Extracellular vesicles (EVs) are lipid bilayer particles naturally released from most if not all cell types to mediate inter-cellular exchange of bioactive molecules. Mounting evidence suggest their important role in diverse pathophysiological processes in the development, growth, homeostasis, and disease. Thus, sensitive and reliable assessments of functional EV cargo transfer from donor to acceptor cells are extremely important. Here, we summarize the methods EV are labeled and their functional transfer in acceptor cells are evaluated by various reporter systems.

Keywords: EVs visualization; cell–cell communication; exosomes; extracellular vesicle; recipient cells; reporter.

Publication types

Review