Salivary expression of lncRNA DQ786243 and IL-17 in oral lichen planus: case-control study

Engy Abdeldayem; Laila Rashed; Shereen Ali

doi:10.1186/s12903-022-02277-0

Salivary expression of lncRNA DQ786243 and IL-17 in oral lichen planus: case-control study

BMC Oral Health. 2022 Jun 18;22(1):240. doi: 10.1186/s12903-022-02277-0.

Authors

Engy Abdeldayem¹, Laila Rashed², Shereen Ali³

Affiliations

¹ Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University, 11 El-Saraya Street, Manial, Cairo, 11553, Egypt. engy_abdeldayem@dentistry.cu.edu.eg.
² Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
³ Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University, 11 El-Saraya Street, Manial, Cairo, 11553, Egypt.

Abstract

Background: A growing number of studies has investigated IL-17 in OLP. However, its exact role and interactions are not fully determined. In addition, the literature investigating its salivary expression is limited. The scarcity in the literature studying lncRNAs was noticed, particularly with regards to correlating them with cytokines in OLP. In the current study, the salivary expression of lncRNA DQ786243 and IL-17 was assessed among different forms of OLP.

Methods: The study included 52 participants in four equal groups: reticular OLP, erythematous OLP, ulcerative OLP, and control group. All eligible OLP patients underwent conventional oral examination, along with basic charting of their demographic data, pain intensity using a visual analogue scale, and clinical evaluation using the Thongprasom et al. scale. The salivary expression of lncRNA DQ786243 and IL-17 was evaluated for all participants using qRT-PCR. Unstimulated whole saliva samples were used. Data were analyzed for statistical significance.

Results: No statistically significant difference was observed when comparing the mean age and gender distribution of the studied groups. A statistically significant difference was detected when comparing pain and clinical scores in the three OLP forms. The highest expression of both salivary biomarkers was noticed in ulcerative OLP, followed by erythematous OLP and reticular OLP, then the controls, with a significant difference between the studied groups. Upon comparing the salivary expression of DQ786243 in ulcerative and erythematous OLP, no significant difference was detected. No significant difference was detected when comparing salivary expression of IL-17 in erythematous OLP to the other OLP forms.

Conclusions: The salivary expression of lncRNA DQ786243 and IL-17 was upregulated in OLP compared to healthy individuals. Besides, their expression increased when the severity of OLP was at its highest level in ulcerative OLP. There was a positive correlation between DQ786243 and IL-17. Trial registration The protocol was registered at ClinicalTrials.gov (NCT04503824). The date of registration is 07/08/2020.

Keywords: IL-17; Oral lichen planus; Saliva; lncRNA DQ786243.

Publication types

Clinical Study
Research Support, Non-U.S. Gov't

MeSH terms

Case-Control Studies
Humans
Interleukin-17 / genetics
Lichen Planus, Oral* / diagnosis
RNA, Long Noncoding* / genetics
Saliva / metabolism

Substances

Interleukin-17
RNA, Long Noncoding

Associated data

ClinicalTrials.gov/NCT04503824