Synergism of deltamethrin with a mixture of short chain fatty acids for toxicity against pyrethroid-resistant and susceptible strains of Tribolium castaneum (Coleoptera: Tenebrionidae)

Abstract

Deltamethrin is one of the most effective pyrethroid compounds used in stored product protection to control a wide range of pests. However, the development of resistance to deltamethrin in many pest species has been reported and useful research to overcome this problem is required. The present study investigated the possible synergistic effect of a commercial formulation of a mixture of the short chain fatty acids, octanoic, nonanoic and decanoic acid, in a formulation called "C8910" on the lethal activity of deltamethrin against susceptible (Lab-S) and relatively pyrethroid-resistant (Pyr-R) strains of T. castaneum. The possible mechanisms of synergism were studied by investigating the inhibitory effect of C8910 on the activity of detoxification enzymes including cytochrome P450s, esterases, and glutathione S-transferases (GST). In addition, the possible role of C8910 in enhancement of cuticular penetration of deltamethrin through insect cuticle was studied using GC analysis. The results showed that C8910 enhanced the toxicity of deltamethrin at mixing ratios of 1:5 and 1:10 against the Lab-S strain after 24 and 48 h of exposure, and synergistic factors (SF) ranged between 5.69 and 13.59. C8910 also showed greater synergism on the deltamethrin toxicity against the resistant strain than the susceptible one after 24 and 48 h of treatment at 1:5 and 1:10 ratios with SF values ranging from 22.82 and 47.16. C8910 showed strong inhibition of cytochrome P450 of rat microsomal fraction with IC₅₀ value of 6.24 mM. Meanwhile, C8910 inhibited the activity of general esterases in Lab-S and Pyr-R strains with IC₅₀ values of 26.22 and 51.73 mM, respectively. However, weak inhibition of GST activity was observed with inhibition of 52.0 and 22.6% at concentration of 100 mM of C8910 for Lab-S and Pyr-R, respectively. In addition, the results showed no significant difference between the unpenetrated amounts of deltamethrin when insects were treated with deltamethrin alone or with deltamethrin+C8910 (1:20) through the insect cuticle. Results suggested that the synergism between C8910 and deltamethrin could be related to the ability of C8910 to inhibit the detoxification enzymes such as cytochrome P450 and esterases. Therefore, C8910 could be a promising synergist to enhance deltamethrin toxicity and to be a possible natural alternative for conventional synergists such as piperonyl butoxide.

Keywords: Cytochrome P450; Esterases; Insecticide resistance; Residual insecticide; Stored product insects; Tribolium flour beetles.