LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

Yuan Li; Yilin Qian; Xiaowei Lou; Zhiheng Hu; Yaqin Hu; Mingyong Zeng; Zunying Liu

doi:10.3389/fmicb.2022.892788

LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach

Front Microbiol. 2022 May 31:13:892788. doi: 10.3389/fmicb.2022.892788. eCollection 2022.

Authors

Yuan Li^{1

2

3}, Yilin Qian^{1

3}, Xiaowei Lou⁴, Zhiheng Hu^{2

5}, Yaqin Hu⁵, Mingyong Zeng^{1

3}, Zunying Liu^{1

3}

Affiliations

¹ College of Food Science and Engineering, Ocean University of China, Qingdao, China.
² College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
³ Qingdao Engineering Research Center for Preservation Technology of Marine Foods, Qingdao, China.
⁴ Department of Food Science and Technology, National University of Singapore, Singapore, Singapore.
⁵ College of Food Science and Technology, Hainan Tropical Ocean University, Sanya, China.

Abstract

This study illustrated the texture changes of Shewanella baltica-inoculated Litopenaeus vannamei during refrigerated storage with the exogenous addition of Lactobacillus plantarum SS-128. The group inoculated with SS-128 had an improved texture compared with that inoculated with the luxS-mutant group (ΔluxS). Proteomics were conducted to analyze the protein alterations in L. vannamei and supernatant, respectively. During storage, many texture-related proteins, including myosin heavy chain and beta-actin, were maintained due to luxS. Some endogenous enzymes related to the energy metabolism and hydrolysis of L. vannamei were downregulated. The luxS-induced interaction with S. baltica showed significant changes in the expression of some critical enzymes and pathways. The ATP-dependent zinc metalloprotease FtsH and protease subunit HslV were downregulated, and the oxidative phosphorylation and glycosaminoglycan degradation pathways in S. baltica were inhibited, resulting in the slow deterioration of L. vannamei. By exploring the mechanism underlying SS-128-led manipulation of the metabolism of spoilage bacteria, we clarified the texture maintenance mechanism of luxS in SS-128, providing theoretical evidence for SS-128 application in food preservation.

Keywords: Lactobacillus plantarum SS-128; Litopenaeus vannamei; luxS; proteomics; texture.