Genome-edited ATP BINDING CASSETTE B1 transporter SD8 knockouts show optimized rice architecture without yield penalty

Ruihong Qu; Pingxian Zhang; Qing Liu; Yifan Wang; Weijun Guo; Zhuoying Du; Xiulan Li; Liwen Yang; Shuangyong Yan; Xiaofeng Gu

doi:10.1016/j.xplc.2022.100347

Genome-edited ATP BINDING CASSETTE B1 transporter SD8 knockouts show optimized rice architecture without yield penalty

Plant Commun. 2022 Sep 12;3(5):100347. doi: 10.1016/j.xplc.2022.100347. Epub 2022 Jun 10.

Authors

Ruihong Qu¹, Pingxian Zhang², Qing Liu³, Yifan Wang¹, Weijun Guo¹, Zhuoying Du¹, Xiulan Li¹, Liwen Yang⁴, Shuangyong Yan⁵, Xiaofeng Gu⁶

Affiliations

¹ Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China.
² College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 Hubei, China.
³ College of Life Sciences, Hebei Agricultural University, Baoding, 071001 Hebei, China.
⁴ Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China. Electronic address: yangliwen@caas.cn.
⁵ Tianjin Key Laboratory of Crop Genetics and Breeding, Tianjin Crop Research Institute, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China. Electronic address: bioysy@139.com.
⁶ Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China. Electronic address: guxiaofeng@caas.cn.

Abstract

This study reports the identification of the rice open reading frame Semi-Dwarf in chr8 (SD8) that encodes a putative ortholog of Arabidopsis thaliana ABCB1. Genome editing of SD8 leads to optimized rice architecture by reducing plant height and flag-leaf angle without yield penalty. Rice SD8 knockouts may also have the potential for increased yield under high density planting.

Keywords: ABCB1; SD8; dwarf; genome editing; leaf angle; rice.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

ATP-Binding Cassette Transporters / genetics
ATP-Binding Cassette Transporters / metabolism
Adenosine Triphosphate / metabolism
Arabidopsis* / genetics
Oryza* / genetics
Oryza* / metabolism
Plant Leaves / genetics

Substances

ATP-Binding Cassette Transporters
Adenosine Triphosphate