Dual nanozyme based on ultrathin 2D conductive MOF nanosheets intergraded with gold nanoparticles for electrochemical biosensing of H2O2 in cancer cells

Wei Huang; Yun Xu; Zhanpeng Wang; Kin Liao; Yan Zhang; Yimin Sun

doi:10.1016/j.talanta.2022.123612

Dual nanozyme based on ultrathin 2D conductive MOF nanosheets intergraded with gold nanoparticles for electrochemical biosensing of H₂O₂ in cancer cells

Talanta. 2022 Nov 1:249:123612. doi: 10.1016/j.talanta.2022.123612. Epub 2022 May 27.

Authors

Wei Huang¹, Yun Xu¹, Zhanpeng Wang², Kin Liao³, Yan Zhang⁴, Yimin Sun⁵

Affiliations

¹ Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, 430074, China.
² Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan, 430022, China.
³ Department of Aerospace Engineering, Khalifa University of Science and Technology, P. O. Box 127788, Abu Dhabi, United Arab Emirates.
⁴ Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430022, China.
⁵ Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan, 430022, China. Electronic address: ymsun@wit.edu.cn.

PMID: 35688080
DOI: 10.1016/j.talanta.2022.123612

Abstract

The development of facile, rapid and cost-effective strategies for sensitive detection of cancer biomarkers in human samples is of great significance for early diagnosis of malignant tumors related diseases. In this work, we develop a high-performance electrochemical biosensor based on highly active dual nanozyme amplified system, i.e., ultrathin two-dimension (2D) conductive metal-organic framework (C-MOF) nanosheets (NSs) decorated with high-density ultrafine gold nanoparticles (Au-NPs), and explore its application in sensitive detection of cancer biomarker H₂O₂ in live cells. The C-MOF NSs {i.e., Cu-HHTP (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene)-NSs} provide large surface area and abundant active open metal sites (Cu-O₄), which could improve the catalytic activity of Cu-HHTP-NSs towards H₂O₂. Moreover, abundant exposed O atoms also serve as anchor sites for the deposition of high-density ultrafine Au-NPs (∼3 nm) without agglomeration. Owing to the synergistic contributions of high catalytic activity of Cu-HHTP-NSs and Au-NPs as well as their unique structural and electrical properties, the as-prepared nanohybrid modified electrode exhibits good sensing performances to H₂O₂ with an extremely low detection limit of 5.6 nM (3σ rules) and a high sensitivity of 188.1 μA cm^-2 mM^-1. Furthermore, the proposed nanozymatic electrochemical biosensor has been applied in real-time tracking H₂O₂ released from different human colon cells to identify colon cancer cells from normal colon epithelial cell, which demonstrates its great prospect for early diagnosis and management of various cancer diseases.

Keywords: 2D conductive metal–organic framework nanosheets; Cancer biomarker detection; Dual nanozyme; Electrochemical biosensor; Gold nanoparticles.

MeSH terms

Biosensing Techniques* / methods
Electrochemical Techniques / methods
Gold / chemistry
Humans
Hydrogen Peroxide
Limit of Detection
Metal Nanoparticles* / chemistry
Metal-Organic Frameworks*
Neoplasms* / diagnosis

Substances

Metal-Organic Frameworks
Gold
Hydrogen Peroxide