Lectin-Mediated Coimmobilization of Cascade Glycoenzymes

Yifei Zhang; Yunzhenshan Gao; You Yong

doi:10.1007/978-1-0716-2269-8_12

Lectin-Mediated Coimmobilization of Cascade Glycoenzymes

Methods Mol Biol. 2022:2487:189-195. doi: 10.1007/978-1-0716-2269-8_12.

Authors

Yifei Zhang^{1

2}, Yunzhenshan Gao³, You Yong⁴

Affiliations

¹ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China. yifeizhang@mail.buct.edu.cn.
² Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China. yifeizhang@mail.buct.edu.cn.
³ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China.
⁴ Department of Chemical Engineering, Tsinghua University, Beijing, China.

PMID: 35687237
DOI: 10.1007/978-1-0716-2269-8_12

Abstract

As the vast majority of enzymes are glycosylated, lectins can serve as molecular glues to agglutinate multiple glycoenzymes for preparing multienzyme catalysts in an efficient and biocompatible way. Taking glucose oxidase and horseradish peroxidase as a model cascade, we describe in this protocol the coimmobilization of cascade glycoenzymes through lectin-mediated protein agglutination with and without magnetic nanoparticles as carriers.

Keywords: ConA-coated magnetic nanoparticles; Concanavalin A; Glycoenzyme; Lectin; Multienzyme catalyst.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Concanavalin A
Enzymes, Immobilized* / metabolism
Glucose Oxidase / metabolism
Horseradish Peroxidase
Lectins* / metabolism

Substances

Enzymes, Immobilized
Lectins
Concanavalin A
Glucose Oxidase
Horseradish Peroxidase