Advances in high-resolution mass spectrometry techniques for analysis of high mass-to-charge ions

Yin-Hung Lai; Yi-Sheng Wang

doi:10.1002/mas.21790

Advances in high-resolution mass spectrometry techniques for analysis of high mass-to-charge ions

Mass Spectrom Rev. 2023 Nov-Dec;42(6):2426-2445. doi: 10.1002/mas.21790. Epub 2022 Jun 9.

Authors

Yin-Hung Lai^{1

2

3}, Yi-Sheng Wang¹

Affiliations

¹ Genomics Research Center, Academia Sinica, Taipei, Taiwan, R.O.C.
² Department of Chemical Engineering, National United University, Miaoli, Taiwan, R.O.C.
³ Institute of Food Safety and Health Risk Assessment, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C.

PMID: 35686331
DOI: 10.1002/mas.21790

Abstract

A major challenge in modern mass spectrometry (MS) is achieving high mass resolving power and accuracy for precision analyses in high mass-to-charge (m/z) regions. To advance the capability of MS for increasingly demanding applications, understanding limitations of state-of-the-art techniques and their status in applied sciences is essential. This review summarizes important instruments in high-resolution mass spectrometry (HRMS) and related advances to extend their working range to high m/z regions. It starts with an overview of HRMS techniques that provide adequate performance for macromolecular analysis, including Fourier-transform, time-of-flight (TOF), quadrupole-TOF, and related data-processing techniques. Methodologies and applications of HRMS for characterizing macromolecules in biochemistry and material sciences are summarized, such as top-down proteomics, native MS, drug discovery, structural virology, and polymer analyses.

Keywords: Fourier-transform; high mass-to-charge; high-resolution mass spectrometry; native MS; quadrupole; time-of-flight; top-down proteomics.

Publication types

Review