Application of Dual-Enhanced Surface-Enhanced Raman Scattering Probe Technology in the Diagnosis of Tumor Cells in Vitro

Yinping Zhao; Yawei Kong; Liwen Chen; Han Sheng; Yiyan Fei; Lan Mi; Bei Li; Jiong Ma

doi:10.3390/molecules27113582

Application of Dual-Enhanced Surface-Enhanced Raman Scattering Probe Technology in the Diagnosis of Tumor Cells in Vitro

Molecules. 2022 Jun 2;27(11):3582. doi: 10.3390/molecules27113582.

Authors

Yinping Zhao¹, Yawei Kong², Liwen Chen², Han Sheng¹, Yiyan Fei², Lan Mi^{1

2}, Bei Li³, Jiong Ma^{1

2

4}

Affiliations

¹ Institute of Biomedical Engineering and Technology, Academy for Engineering and Technology, Fudan University, Shanghai 200433, China.
² Department of Optical Science and Engineering, School of Information Science and Technology, Fudan University, Shanghai 200433, China.
³ State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Changchun 130000, China.
⁴ Shanghai Engineering Research Center of Industrial Microorganisms, The Multiscale Research Institute of Complex Systems (MRICS), School of Life Sciences, Fudan University, Shanghai 200433, China.

Abstract

With the development of precision medicine, antigen/antibody-targeted therapy has brought great hope to tumor patients; however, the migration of tumor cells, especially a small number of cells flowing into blood or other tissues, remains a clinical challenge. In particular, it is difficult to use functional gold nanomaterials for targeted clinical tumor diagnosis while simultaneously obtaining stable and highly sensitive Raman signals. Therefore, we developed a detection method for functional Au Nanostars (AuNSs) with dual signal enhancement that can specifically track location and obtain high-intensity surface-enhanced Raman scattering (SERS) signals. First, AuNSs with specific optical properties were synthesized and functionalized. The Raman dye 4-mercapto-hydroxybenzoic acid and polyethylene glycol were coupled with the tumor marker, epidermal growth factor receptor, to obtain the targeted SERS probes. In addition, a detection chip was prepared for Raman detection with physical enhancement, exhibiting a 40-times higher signal intensity than that of quartz glass. This study combines physical enhancement and SERS enhancement technologies to achieve dual enhancement, enabling the detection of a highly sensitive and stable Raman signal; this has potential clinical value for antigen/antibody-targeted tumor diagnosis and treatment.

Keywords: A549 cell; Raman signals; SERS probe; dual enhancement; stable and highly sensitive.

MeSH terms

Cell Count
Gold
Humans
Metal Nanoparticles*
Nanostructures*
Spectrum Analysis, Raman / methods
Technology

Substances

Gold

Grants and funding

This research was funded by the National Key R&D Program of China (2021YFF0502900); National Natural Science Foundation of China (62175034, 62175036, 82030106); Shanghai Natural Science Foundation (20ZR1405100, 20ZR1403700); Science and Technology Research Program of Shanghai (19DZ2282100); Shanghai Key Discipline Construction Plan (2020–2022) (GWV−10.1−XK01); Shanghai Engineering Technology Research Center of Hair Medicine (19DZ2250500); Medical Engineering Fund of Fudan University (yg2021−022); Pioneering Project of Academy for Engineering and Technology, Fudan University (gyy2018−001, gyy2018−002); and Yantai Returned Scholars’ Pioneering Park.