α-Viniferin and ε-Viniferin Inhibited TGF-β1-Induced Epithelial-Mesenchymal Transition, Migration and Invasion in Lung Cancer Cells through Downregulation of Vimentin Expression

Wei-Chung Chiou; Cheng Huang; Zi-Jun Lin; Lian-Sheng Hong; Yu-Heng Lai; Jui-Chieh Chen; Hsiu-Chen Huang

doi:10.3390/nu14112294

α-Viniferin and ε-Viniferin Inhibited TGF-β1-Induced Epithelial-Mesenchymal Transition, Migration and Invasion in Lung Cancer Cells through Downregulation of Vimentin Expression

Nutrients. 2022 May 30;14(11):2294. doi: 10.3390/nu14112294.

Authors

Wei-Chung Chiou¹, Cheng Huang¹, Zi-Jun Lin^{2

3}, Lian-Sheng Hong^{2

3}, Yu-Heng Lai⁴, Jui-Chieh Chen⁵, Hsiu-Chen Huang^{2

3}

Affiliations

¹ Laboratory Science in Medicine, Department of Biotechnology, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan.
² Center for Teacher Education, National Tsing Hua University, Hsinchu 300, Taiwan.
³ Department of Applied Science, Nanda Campus, National Tsing Hua University, Hsinchu 300, Taiwan.
⁴ Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan.
⁵ Department of Biochemical Science and Technology, National Chiayi University, Chiayi City 60004, Taiwan.

Abstract

Resveratrol has well-known anticancer properties; however, its oligomers, including α-viniferin, ε-viniferin, and kobophenol A, have not yet been well investigated. This is the first study examining the anti-epithelial-mesenchymal transition (EMT) effects of α-viniferin and ε-viniferin on A549, NCI-H460, NCI-H520, MCF-7, HOS, and U2OS cells. The results showed that α-viniferin and ε-viniferin significantly inhibited EMT, invasion and migration in TGF-β1- or IL-1β-induced non-small cell lung cancer. α-Viniferin and ε-viniferin also reversed TGF-β1-induced reactive oxygen species (ROS), MMP2, vimentin, Zeb1, Snail, p-SMAD2, p-SMAD3, and ABCG2 expression in A549 cells. Furthermore, ε-viniferin was found to significantly inhibit lung metastasis in A549 cell xenograft metastatic mouse models. In view of these findings, α-viniferin and ε-viniferin may play an important role in the prevention of EMT and cancer metastasis in lung cancer.

Keywords: TGF-β1; epithelial-mesenchymal transition; nude mice; vimentin; α-viniferin; ε-viniferin.

MeSH terms

Animals
Benzofurans
Carcinoma, Non-Small-Cell Lung* / drug therapy
Cell Line, Tumor
Cell Movement
Down-Regulation
Epithelial-Mesenchymal Transition
Humans
Lung Neoplasms* / pathology
Mice
Stilbenes
Transforming Growth Factor beta1 / metabolism
Vimentin / genetics
Vimentin / metabolism

Substances

Benzofurans
Stilbenes
Transforming Growth Factor beta1
Vimentin
epsilon-viniferin
alpha-viniferin

Grants and funding

This work was supported by the Ministry of Science and Technology, Taiwan under grant no. MOST 108-2320-B-007-006-MY3.