Evaluation of the Microbiological Effectiveness of Three Accessible Mask Decontamination Methods and Their Impact on Filtration, Air Permeability and Physicochemical Properties

Int J Environ Res Public Health. 2022 May 27;19(11):6567. doi: 10.3390/ijerph19116567.

Abstract

The need to secure public health and mitigate the environmental impact associated with the massified use of respiratory protective devices (RPD) has been raising awareness for the safe reuse of decontaminated masks by individuals and organizations. Among the decontamination treatments proposed, in this work, three methods with the potential to be adopted by households and organizations of different sizes were analysed: contact with nebulized hydrogen peroxide (H2O2); immersion in commercial bleach (NaClO) (sodium hypochlorite, 0.1% p/v); and contact with steam in microwave steam-sanitizing bags (steam bag). Their decontamination effectiveness was assessed using reference microorganisms following international standards (issued by ISO and FDA). Furthermore, the impact on filtration efficiency, air permeability and several physicochemical and structural characteristics of the masks, were evaluated for untreated masks and after 1, 5 and 10 cycles of treatment. Three types of RPD were analysed: surgical, KN95, and cloth masks. Results demonstrated that the H2O2 protocol sterilized KN95 and surgical masks (reduction of >6 log10 CFUs) and disinfected cloth masks (reduction of >3 log10 CFUs). The NaClO protocol sterilized surgical masks, and disinfected KN95 and cloth masks. Steam bags sterilized KN95 and disinfected surgical and cloth masks. No relevant impact was observed on filtration efficiency.

Keywords: air permeability; commercial bleach; decontamination methods; filtration efficiency; microbiological effectiveness; microwave steam-sanitizing bag; nebulized hydrogen peroxide; physicochemical properties; respiratory protective devices (RPD).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Decontamination* / methods
  • Filtration
  • Humans
  • Hydrogen Peroxide
  • Permeability
  • Respiratory Protective Devices*
  • Steam

Substances

  • Steam
  • Hydrogen Peroxide

Grants and funding

This research was funded by the Portuguese Foundation for Science and Technology (FCT) through project nº 395—“Evaluation of the Effectiveness of Decontamination, and Safety of Reuse the Respiratory Protective Devices” (2nd edition of RESEARCH4COVID 19), and contract Ref. UIDB/EQU/00102/2020. A.M.A. Dias acknowledges FCT-MEC support, through contract with Ref. CEECIND/01248/2017.