Advanced functional fluorescent probes for cell plasma membranes

Mayeul Collot; Sonia Pfister; Andrey S Klymchenko

doi:10.1016/j.cbpa.2022.102161

Advanced functional fluorescent probes for cell plasma membranes

Curr Opin Chem Biol. 2022 Aug:69:102161. doi: 10.1016/j.cbpa.2022.102161. Epub 2022 Jun 5.

Authors

Mayeul Collot¹, Sonia Pfister², Andrey S Klymchenko³

Affiliations

¹ Laboratoire de Bioimagerie et Pathologies, UMR 7021, CNRS/Université de Strasbourg, 74 route du Rhin, 67401, Illkirch-Graffenstaden, France. Electronic address: mayeul.collot@unistra.fr.
² Laboratoire de Bioimagerie et Pathologies, UMR 7021, CNRS/Université de Strasbourg, 74 route du Rhin, 67401, Illkirch-Graffenstaden, France.
³ Laboratoire de Bioimagerie et Pathologies, UMR 7021, CNRS/Université de Strasbourg, 74 route du Rhin, 67401, Illkirch-Graffenstaden, France. Electronic address: andrey.klymchenko@unistra.fr.

PMID: 35675723
DOI: 10.1016/j.cbpa.2022.102161

Abstract

Imaging the plasma membrane (PM) by fluorescence techniques using molecular fluorescent probes enable cell segmentation, studying membrane organization and dynamics, formation, and tracking of vesicles. Rational molecular design brings fluorescent PM probes to a new level, providing PM probes with new functions beyond basic PM staining and imaging. We herein review the latest advances in fluorescent PM probes for chemical and biophysical sensing as well as for super-resolution imaging.

Keywords: Fluorescence microscopy; Fluorescent probes; Lipid probes; Plasma membrane probes; Probing cell surface; SMLM; STED; Super resolution.

Publication types

Review
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cell Membrane / metabolism
Fluorescent Dyes* / chemistry
Microscopy, Fluorescence / methods
Molecular Probes* / metabolism

Substances

Fluorescent Dyes
Molecular Probes