COL1A1 and FGFR2 Single-Nucleotide Polymorphisms Found in Class II and Class III Skeletal Malocclusions in Javanese Population

I Gusti Aju Wahju Ardani; Melisa Budipramana; Erlina Rachmawati; Alexander Patera Nugraha; I Kade Karisma Gita Ardana; Theresia Indah Budhy; Rozita Hassan; Dwi Listyorini; Riyanarto Sarno

doi:10.1055/s-0042-1744371

COL1A1 and FGFR2 Single-Nucleotide Polymorphisms Found in Class II and Class III Skeletal Malocclusions in Javanese Population

Eur J Dent. 2023 Feb;17(1):183-190. doi: 10.1055/s-0042-1744371. Epub 2022 Jun 7.

Authors

I Gusti Aju Wahju Ardani¹, Melisa Budipramana², Erlina Rachmawati¹, Alexander Patera Nugraha¹, I Kade Karisma Gita Ardana³, Theresia Indah Budhy⁴, Rozita Hassan⁵, Dwi Listyorini⁶, Riyanarto Sarno⁷

Affiliations

¹ Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
² Department of Orthodontics, Faculty of Dental Medicine, Universitas Lambung Mangkurat, Banjarmasin, Indonesia.
³ Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia.
⁴ Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
⁵ Orthodontic Unit, School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
⁶ Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia.
⁷ Department of Informatics, Institute Technology of Sepuluh Nopember, Surabaya, Indonesia.

Abstract

Objective: The aim of this article is to analyze and compare the presence of single-nucleotide polymorphisms (SNPs) of COL1A1 and FGFR2 in class II and class III Javanese populations.

Materials and methods: Cephalometric radiographs from total 63 patients of class II and III were analyzed. SNP analysis was performed based on both COL1A1 and FGFR2 sequences amplified from total DNA of patients' fresh blood. Principal component analysis was done to calculate the data and find the correlation of the cephalometric indicators influenced by each mutation. t-test and Mann-Whitney analysis were performed to check the significance of differences occurred in each studied parameter (p < 0.05).

Result: There were three COL1A1 SNPs found in class II and only two in class III, while three FGFR2 SNPs found in both classes. Class II phenotype seemed to be strongly influenced by Y-axis and mandibular plane angle, while class III by lower gonial angle and mandibular plane angle.

Conclusion: Based on this study, we suggest that rs2249492 of COL1A1 and rs2981582 of FGFR2 play important roles in class III, while rs2277632 of COL1A1 and rs2981582 of FGFR2 play important role in class II skeletal malocclusion in Javanese population.

The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).