Stability of a half-sandwich Os(II) complex with indomethacin-functionalized ligand in the presence of carboxypeptidase A

Lukáš Masaryk; Darina Muthná; Petr Halaš; Pavel Zoufalý; Eva Peterová; Radim Havelek; Bohuslav Drahoš; David Milde; Alena Mrkvicová; Pavel Štarha

doi:10.1039/d2dt01085b

Stability of a half-sandwich Os(II) complex with indomethacin-functionalized ligand in the presence of carboxypeptidase A

Dalton Trans. 2022 Jun 21;51(24):9213-9217. doi: 10.1039/d2dt01085b.

Authors

Affiliations

¹ Department of Inorganic Chemistry, Faculty of Science, Palacky University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic. pavel.starha@upol.cz.
² Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Šimkova 870, 500 03 Hradec Kralove, Czech Republic.
³ Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic.

PMID: 35670076
DOI: 10.1039/d2dt01085b

Abstract

In the presence of carboxypeptidase, the hydrolytically stable complex [Os(η⁶-pcym)(L2)Cl]PF₆ (2) partially released the bioactive substituent indomethacin, bound through the amide bond to the chelating 2-(1,3,4-thiadiazol-2-yl)pyridine-based moiety of L2. Stability in the presence of other relevant biomolecules (GSH, NADH, GMP) and cancer cell viability were also studied.

MeSH terms

Antineoplastic Agents* / chemistry
Carboxypeptidases A
Cell Line, Tumor
Coordination Complexes* / chemistry
Coordination Complexes* / pharmacology
Indomethacin / pharmacology
Ligands

Substances

Antineoplastic Agents
Coordination Complexes
Ligands
Carboxypeptidases A
Indomethacin