Present study aimed to explore the antihypertensive potential of bioactive peptides isolated from Brassica napus protein as inhibitor of angiotensin converting enzyme. Protein was extracted and assessed for antihypertensive potential. The extracted protein showed 72% antihypertensive activity/potential with IC50 value of 24±5.60μg/mL. Thirty-one fractions of peptides were isolated by hydrolyzing protein at different time intervals, pH, temperature and enzyme/substrate ratio. The antihypertensive potential of all isolated fractions was measured. It was found that only one peptide fraction exhibited significantly high (75%) antihypertensive potential. This hydrolyzed fraction was characterized through Liquid-Chromatography-Electrospray-Ionization-Mass-Spectrometry (LC-ESI-MS/MS). Eleven bioactive peptides were identified in hydrolyzate of Brassica napus which include Serine-Threonine, Methionine-Valine, Methionine-Leucine, Glutamine-Phenylalanine, Alanine-Threonine-Phenylalanine, Alanine-Leucine-Proline-Glycine, Valine-Alanine-Phenylalanine-Glycine, Aspartic acid-Proline-Methionine-Glutamine, Valine-Glutamine-Cysteine-Tyrosine, Methionine-Cysteine-Tyrosine-Tyrosine-Phenylalanine and Alanine-Leucine-Leucine-Alanine-Cysteine-Proline-Alanine. The current study showed that Brassica napus is an important food, having high amount of bioactive peptides with high antihypertensive potential, can control blood pressure very efficiently.