Determination of glyphosate and AMPA in freshwater and soil from agroecosystems by 9-fluorenylmethoxycarbonyl chloride derivatization and liquid chromatography - fluorescence detection and tandem mass spectrometry

Beatriz Alonso; Luciana Griffero; Heinkel Bentos Pereira; Lucía Pareja; Andrés Pérez Parada

doi:10.1016/j.mex.2022.101730

Determination of glyphosate and AMPA in freshwater and soil from agroecosystems by 9-fluorenylmethoxycarbonyl chloride derivatization and liquid chromatography - fluorescence detection and tandem mass spectrometry

MethodsX. 2022 May 13:9:101730. doi: 10.1016/j.mex.2022.101730. eCollection 2022.

Authors

Beatriz Alonso¹, Luciana Griffero¹, Heinkel Bentos Pereira¹, Lucía Pareja², Andrés Pérez Parada¹

Affiliations

¹ Departamento de Desarrollo Tecnológico, Centro Universitario Regional del Este, Universidad de la República, Uruguay.
² Departamento de Química del Litoral, Centro Universitario Regional Litoral Norte, Universidad de la República, Uruguay.

Abstract

The herbicide glyphosate (GLY) and its metabolite aminophosphonic acid (AMPA) are troublesome compounds for analysis in the environment. Here we report a reliable technique for GLY and AMPA determination in freshwater and soils by means of derivatization with 9-fluorenylmethoxycarbonyl chloride (FMOC-Cl) and further liquid chromatography with fluorescence detection (FLD) and tandem mass spectrometry (MS/MS) analysis. Selected experiments were carried out to evaluate selectivity, sensitivity, repeatability, linearity and quantification performance in both matrices.

Keywords: AMPA; FMOC-Cl; environmental fate of pesticides; fluorescence detection (FLD); glyphosate; liquid chromatography (LC); tandem mass spectrometry (MS/MS).