Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Piaoye Ming; Pengcheng Rao; Tianli Wu; Jianghua Yang; Shi Lu; Binbin Yang; Jingang Xiao; Gang Tao

doi:10.3389/fbioe.2022.899293

Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration

Front Bioeng Biotechnol. 2022 May 19:10:899293. doi: 10.3389/fbioe.2022.899293. eCollection 2022.

Authors

Piaoye Ming^{1

2}, Pengcheng Rao^{1

3}, Tianli Wu^{1

2}, Jianghua Yang¹, Shi Lu^{1

2}, Binbin Yang¹, Jingang Xiao^{1

2

3}, Gang Tao¹

Affiliations

¹ Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, China.
² Department of Oral Implantology, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, China.
³ Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Abstract

The guided tissue regeneration (GTR) technique is a promising treatment for periodontal tissue defects. GTR membranes build a mechanical barrier to control the ingrowth of the gingival epithelium and provide appropriate space for the regeneration of periodontal tissues, particularly alveolar bone. However, the existing GTR membranes only serve as barriers and lack the biological activity to induce alveolar bone regeneration. In this study, sericin-hydroxyapatite (Ser-HAP) composite nanomaterials were fabricated using a biomimetic mineralization method with sericin as an organic template. The mineralized Ser-HAP showed excellent biocompatibility and promoted the osteogenic differentiation of human periodontal membrane stem cells (hPDLSCs). Ser-HAP was combined with PVA using the freeze/thaw method to form PVA/Ser-HAP membranes. Further studies confirmed that PVA/Ser-HAP membranes do not affect the viability of hPDLSCs. Moreover, alkaline phosphatase (ALP) staining, alizarin red staining (ARS), and RT-qPCR detection revealed that PVA/Ser-HAP membranes induce the osteogenic differentiation of hPDLSCs by activating the expression of osteoblast-related genes, including ALP, Runx2, OCN, and OPN. The unique GTR membrane based on Ser-HAP induces the differentiation of hPDLSCs into osteoblasts without additional inducers, demonstrating the excellent potential for periodontal regeneration therapy.

Keywords: biomimetic membranes; human periodontal membrane stem cells; nano-hydroxyapatite; osteogenic differentiation; sericin.