Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed-Phase HPLC-MS Under Non-derivatized Conditions

Attila L Ádám; György Kátay; András Künstler; Lóránt Király

doi:10.1007/978-1-0716-2469-2_14

Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed-Phase HPLC-MS Under Non-derivatized Conditions

Methods Mol Biol. 2022:2526:191-200. doi: 10.1007/978-1-0716-2469-2_14.

Authors

Attila L Ádám¹, György Kátay¹, András Künstler¹, Lóránt Király²

Affiliations

¹ Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network (ELKH), Budapest, Hungary.
² Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network (ELKH), Budapest, Hungary. kiraly.lorant@atk.hu.

PMID: 35657521
DOI: 10.1007/978-1-0716-2469-2_14

Abstract

Azelaic acid (AzA, 1,9-nonadienoic acid) is a nine-carbon chain (C₉) dicarboxylic acid with multiple and diverse functions in humans and plants. In plants this compound was suggested as a marker for lipid peroxidation under biotic and abiotic stress conditions and an inducer (priming agent) of plant immunity (acquired resistance). Detection methods for AzA in plants include a wide range of methodological approaches. This new and simple reversed-phase HPLC-MS protocol describes the measurement of AzA and other dicarboxylic acids either from tobacco leaf tissue or petiolar exudates (vascular sap) of plants under non-derivatized conditions.

Keywords: Azelaic acid; Biotic stress; Dicarboxylic acids; HPLC-MS; Lipid peroxidation; Plant immunity; Tobacco; Tobacco mosaic virus (TMV).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers
Chromatography, High Pressure Liquid
Dicarboxylic Acids*
Humans
Lipid Peroxidation
Nicotiana*
Plant Diseases
Stress, Physiological

Substances

Biomarkers
Dicarboxylic Acids
azelaic acid