Towards identification of protein-protein interaction stabilizers via inhibitory peptide-fragment hybrids using templated fragment ligation

Sonja Srdanović; Zsofia Hegedüs; Stuart L Warriner; Andrew J Wilson

doi:10.1039/d2cb00025c

Towards identification of protein-protein interaction stabilizers via inhibitory peptide-fragment hybrids using templated fragment ligation

RSC Chem Biol. 2022 Apr 1;3(5):546-550. doi: 10.1039/d2cb00025c. eCollection 2022 May 11.

Authors

Sonja Srdanović^{1

2}, Zsofia Hegedüs^{1

2

3}, Stuart L Warriner^{1

2}, Andrew J Wilson^{1

2}

Affiliations

¹ Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane Leeds LS2 9JT UK a.j.wilson@leeds.ac.uk.
² School of Chemistry, University of Leeds, Woodhouse Lane Leeds LS2 9JT UK.
³ Department of Medical Chemistry, University of Szeged Dóm tér 8 H-6720 Szeged Hungary.

Abstract

Using the hDMX/14-3-3 interaction, acylhydrazone-based ligand-directed fragment ligation was used to identify protein-protein interaction (PPI) inhibitory peptide-fragment hybrids. Separation of the peptide-fragment hybrids into the components yielded fragments that stabilized the hDMX/14-3-3 interaction.