l-Asparaginase Encapsulation into Asymmetric Permeable Polymersomes

Cecilia Z Bueno; Alexsandra C Apolinário; Aroa Duro-Castano; Alessandro Poma; Adalberto Pessoa Jr; Carlota O Rangel-Yagui; Giuseppe Battaglia

doi:10.1021/acsmacrolett.0c00619

l-Asparaginase Encapsulation into Asymmetric Permeable Polymersomes

ACS Macro Lett. 2020 Oct 20;9(10):1471-1477. doi: 10.1021/acsmacrolett.0c00619. Epub 2020 Oct 9.

Authors

Cecilia Z Bueno^{1

2}, Alexsandra C Apolinário^{1

3

2}, Aroa Duro-Castano², Alessandro Poma^{2

4}, Adalberto Pessoa Jr¹, Carlota O Rangel-Yagui¹, Giuseppe Battaglia^{2

5

6}

Affiliations

¹ Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 São Paulo, Brazil.
² Department of Chemistry, University College London, WC1H 0AJ London, United Kingdom.
³ Department of Pharmacology, University of São Paulo, 05508-000 São Paulo, Brazil.
⁴ Eastman Dental Institute, University College London, WC1X 8LD London, United Kingdom.
⁵ Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), 08028 Barcelona, Spain.
⁶ Catalan Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain.

PMID: 35653665
DOI: 10.1021/acsmacrolett.0c00619

Abstract

This work reports, for the encapsulation of l-asparaginase, an anticancer enzyme into hybrid PMPC₂₅-PDPA₇₀/PEO₁₆-PBO₂₂ asymmetric polymersomes previously developed by our group, with loading capacities with over 800 molecules per vesicle. Enzyme-loaded polymersomes show permeability and capacity to hydrolyze l-asparagine, which is essential to cancer cells. The nanoreactors proposed in this work can be potentially used in further studies to develop novel therapeutic alternatives based on l-asparaginase.