Structural Characterization of Gracilariopsis lemaneiformis Polysaccharide and Its Property in Delaying Cellular Senescence

Xiaomei Wang; Xiaogang Xu; Genxiang Mao; Yue Guo; Guangce Wang; Xue Sun; Nianjun Xu; Zhongshan Zhang

doi:10.3389/fnut.2022.876992

Structural Characterization of Gracilariopsis lemaneiformis Polysaccharide and Its Property in Delaying Cellular Senescence

Front Nutr. 2022 May 16:9:876992. doi: 10.3389/fnut.2022.876992. eCollection 2022.

Authors

Xiaomei Wang¹, Xiaogang Xu², Genxiang Mao², Yue Guo¹, Guangce Wang³, Xue Sun⁴, Nianjun Xu⁴, Zhongshan Zhang¹

Affiliations

¹ Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, China.
² Key Lab of Geriatrics & Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou, China.
³ CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
⁴ National Engineering Research Laboratory of Marine Biotechnology and Engineering, Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China.

Abstract

The sulfated polysaccharide was isolated from the purified G. lemaneiformis polysaccharide (PGP), and its property in delaying H₂O₂-induced 2BS cellular senescence was studied. The results showed that PGP was a linear polysaccharide containing alternating α-(1 → 3)- and β-(1 → 4)-galactopyranose units. Most of the sulfate groups are at C6 of the -(1 → 4)-α-D-Galp, and a small part of them are at C3 and C6. PGP pretreatment could decrease SA-β-gal-positive cells and prevent the formation of senescence-associated heterochromatic foci (SAHF) induced by H₂O₂ in a dose-dependent manner. It is speculated that PGP may delay aging by downregulating the expression of p21 and p53 genes. The finding provides new insights into the beneficial role of G. lemaneiformis polysaccharide (GP) on retarding senescence process.

Keywords: G. lemaneiformis; SA-β-gal; cellular senescence; p53-p21 pathway; polysaccharide.