Ti3C2@WSe2 as photoelectractive materials coupling with recombinase polymerase amplification for nucleic acid detection

Abstract

The photoelectrochemical biosensor based on double redox cycle amplification technology coupled with Tungsten diselenide and MXene-modified electrode was developed. Signal amplification technology is a commonly used method to improve the sensitivity. In this article, in the double redox cycle amplification method, p-aminophenol is used as the signal molecule, and squaric acid acts as a redox indicator. Tris(2-carboxyethyl) phosphine is an excellent reducing agent, which greatly improves the photoelectric response. Tungsten diselenide and MXene are used as photosensitive materials. In the presence of model target, respiratory syncytial virus RNA, the recombinase polymerase amplification process occurred because there is amplification template, so that the signal molecule p-aminophenol will be produced, leading to redox cycle was carried out, and the photocurrent signal is improved. In the absence of syncytial virus RNA, the photocurrent signal is low. The detection range of the biosensor is from 0.2 fM to 80 fM, and the detection limit reaches 30 aM for respiratory syncytial virus RNA. The method of introducing redox cycle amplification and Tungsten diselenide, MXene into photoelectrochemical biosensing provides a new idea for future biological analysis and has application potential.

Keywords: Double redox cycle; Respiratory syncytial virus; Signal amplification; Ti(3)C(2); WSe(2).