Simultaneous exposure of wheat (Triticum aestivum L.) to CuO and S nanoparticles alleviates toxicity by reducing Cu accumulation and modulating antioxidant response

Abstract

Widespread use of metal-based nanoparticles (NPs) may result in the increased accumulation of metals in agricultural soil, which could affect crop productivity and contaminate the food-chain. The effect of sulfur nanoparticles (S NPs, 200 mg/L) co-exposure on the toxicity of CuO nanoparticles (CuO NPs, 25 and 50 mg/L) to wheat seedlings was investigated in a hydroponic system. CuO NPs exposure significantly inhibited the growth of wheat seedlings, causing 43.6% and 54.1% decreases in the fresh biomass of plants and 82.8% and 83.1% decrease in the total chlorophyll contents at 25 and 50 mg/L (CuONP25 and CuONP50), respectively, as compared to controls. CuO NPs exposure at both concentrations increased the malondialdehyde (MDA) content in shoot and root tissues by 66.4-67.9% and 47.7-48.8%, respectively. Further, CuO NPs exposure elevated the activities POD, SOD, and CAT by 2.19-2.27, 5.82-6.09, and 1.44-1.95 times in roots, and by 45.2-67.8%, 86.7-154.5%, and 22.5-56.1% in shoot, respectively, in comparison to control. The addition of S NPs alone increased wheat biomass by 11.0% and total chlorophyll contents by 4.4%, compared to controls. Further, simultaneous exposure to S NPs (200 mg/L) and CuO NPs (25 or 50 mg/L) alleviated the CuO NPs toxicity; wheat biomass was 47.8% and 37.7% higher in CuONP25 + SNP and CuONP50 + SNP treatments, respectively, as compared to CuO NPs alone treated plants. Co-exposed plants showed reduced levels of total reactive oxygen species (ROS), O₂^·- and H₂O₂. Additionally, S NPs exposure reduced Cu uptake and accumulation in both root and shoot tissue by 32.2-54.4% and 38.3-57.5%, respectively. In summary, S NPs alleviated CuO NPs toxicity to wheat seedlings, most likely by reducing Cu bioavailability and accumulation of Cu in plant tissues, and also altered S nutrition and the modulation of antioxidant response in plants. These results showed that S NPs application has the potential to alleviate CuO NP toxicity and increase wheat productivity affected by metals toxicity.

Keywords: Antioxidant enzyme activity; CuO nanoparticles; Oxidative stress; ROS; Sulfur nanoparticles.