Changes in Lake Sturgeon Gut Microbiomes Relative to Founding Origin and in Response to Chemotherapeutant Treatments

Shairah Abdul Razak; John M Bauman; Terence L Marsh; Kim T Scribner

doi:10.3390/microorganisms10051005

Changes in Lake Sturgeon Gut Microbiomes Relative to Founding Origin and in Response to Chemotherapeutant Treatments

Microorganisms. 2022 May 10;10(5):1005. doi: 10.3390/microorganisms10051005.

Authors

Shairah Abdul Razak^{1

2}, John M Bauman³, Terence L Marsh⁴, Kim T Scribner^{1

5}

Affiliations

¹ Department of Fisheries & Wildlife, Michigan State University, East Lansing, MI 48824, USA.
² Department of Applied Physics, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
³ Michigan Department of Natural Resources Fisheries Division, Escanaba Customer Service Center, Gladstone, MI 49837, USA.
⁴ Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
⁵ Department of Integrative Biology, Michigan State University, East Lansing, MI 48824, USA.

Abstract

Antibiotics, drugs, and chemicals (collectively referred to as chemotherapeutants) are widely embraced in fish aquaculture as important tools to control or prevent disease outbreaks. Potential negative effects include changes in microbial community composition and diversity during early life stages, which can reverse the beneficial roles of gut microbiota for the maintenance of host physiological processes and homeostatic regulation. We characterized the gut microbial community composition and diversity of an ecologically and economically important fish species, the lake sturgeon (Acipenser fulvescens), during the early larval period in response to weekly treatments using chemotherapeutants commonly used in aquaculture (chloramine-T, hydrogen peroxide, and NaCl₂ followed by hydrogen peroxide) relative to untreated controls. The effects of founding microbial community origin (wild stream vs. hatchery water) were also evaluated. Gut communities were quantified using massively parallel next generation sequencing based on the V4 region of the 16S rRNA gene. Members of the phylum Firmicutes (principally unclassified Clostridiales and Clostridium_sensu_stricto) and Proteobacteria were the dominant taxa in all gut samples regardless of treatment. The egg incubation environment (origin) and its interaction with chemotherapeutant treatment were significantly associated with indices of microbial taxonomic diversity. We observed large variation in the beta diversity of lake sturgeon gut microbiota between larvae from eggs incubated in hatchery and wild (stream) origins based on nonmetric dimensional scaling (NMDS). Permutational ANOVA indicated the effects of chemotherapeutic treatments on gut microbial community composition were dependent on the initial source of the founding microbial community. Influences of microbiota colonization during early ontogenetic stages and the resilience of gut microbiota to topical chemotherapeutic treatments are discussed.

Keywords: chemotherapeutants; environmental variation; founder effects; gut microbiome; lake sturgeon.

Grants and funding

1202/Great Lakes Fishery Trust